A simple solid-phase radioimmunoassay for the measurement of IgG secreted in vitro by human lymphocytes

A radioimmunoassay is described for measuring IgG, based on the ability of immunoglobulins of this class to inhibit the binding of radioiodinated staphylococcal protein A to IgG linked to a solid phase. The solid phase is represented by ox erythrocytes coated with anti-ox erythrocyte rabbit IgG, a r...

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Bibliographic Details
Published inJournal of immunological methods Vol. 29; no. 3; p. 263
Main Authors Romagnani, S, Del Prete, G F, Giudizi, G M, Almerigogna, F, Ricci, M
Format Journal Article
LanguageEnglish
Published Netherlands 1979
Subjects
Animals
Binding Sites, Antibody
Binding, Competitive
Cattle
Cells, Cultured
Erythrocytes - immunology
Humans
Immunoglobulin G
Lymphocytes - immunology
Lymphocytes - metabolism
Rabbits
Radioimmunoassay
Receptors, Fc - immunology
Staphylococcal Protein A - immunology
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Summary:A radioimmunoassay is described for measuring IgG, based on the ability of immunoglobulins of this class to inhibit the binding of radioiodinated staphylococcal protein A to IgG linked to a solid phase. The solid phase is represented by ox erythrocytes coated with anti-ox erythrocyte rabbit IgG, a reagent used for detecting cells equipped with receptors for the Fc fragment of IgG. By this assay the IgG secreted in vitro by human peripheral blood lymphocytes stimulated with PWM and those present in samples of very diluted human sera were measured. It was found that the assay is a very rapid, simple and reproducible procedure for the detection of IgG immunoglobulin at the nanogram level.
ISSN:0022-1759
DOI:10.1016/0022-1759(79)90314-4