The Mutation Breeding and Mutagenic Effect of Air Plasma on Penicillium Chrysogenum
Low temperature air plasma was used as the mutation tool for penicillin-producing strain Penicillium chrysogenum. The discharge conditions were RF power of 360 W, temperature of 40℃ in a sealed chamber, and pressure of 10 Pa to 30 Pa. The result showed that the kinetics of the survival rate followed...
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| Published in | Plasma science & technology Vol. 14; no. 4; pp. 297 - 302 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
01.04.2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1009-0630 |
| DOI | 10.1088/1009-0630/14/4/06 |
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| Summary: | Low temperature air plasma was used as the mutation tool for penicillin-producing strain Penicillium chrysogenum. The discharge conditions were RF power of 360 W, temperature of 40℃ in a sealed chamber, and pressure of 10 Pa to 30 Pa. The result showed that the kinetics of the survival rate followed a typical saddle-shaped curve. Based on a statistic analysis, at the treating duration of 10 min, the positive mutation rate was as high as 37.5% while the negative mutation rate was low. The colonial morphology changed obviously when the plasma treating duration reached or exceeded 45 min. After both primary and secondary screening, a mutant designated as aPc051310 with high productivity of penicillin was obtained, and a strong mutagenic effect on P. chrysogenurn was observed in the process. It was proved that after five generations, the mutant aPc051310 still exhibits a high productivity. All the results prove that the plasma mutation method could be developed as a convenient and effective tool to breed high-yield strains in the fermentation industry, while expanding the plasm application at the same time. |
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| Bibliography: | GUI Fang WANG Hui WANG Peng LIU Hui CAI Xiaochun HU Yihua YUAN Chengling ZHENG Zhiming 1Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031 China 2Electronic Engineering Institute, Hefei 230037, China low temperature air plasma, Penicillium chrysogenum, mutation breeding mutation mechanism Low temperature air plasma was used as the mutation tool for penicillin-producing strain Penicillium chrysogenum. The discharge conditions were RF power of 360 W, temperature of 40℃ in a sealed chamber, and pressure of 10 Pa to 30 Pa. The result showed that the kinetics of the survival rate followed a typical saddle-shaped curve. Based on a statistic analysis, at the treating duration of 10 min, the positive mutation rate was as high as 37.5% while the negative mutation rate was low. The colonial morphology changed obviously when the plasma treating duration reached or exceeded 45 min. After both primary and secondary screening, a mutant designated as aPc051310 with high productivity of penicillin was obtained, and a strong mutagenic effect on P. chrysogenurn was observed in the process. It was proved that after five generations, the mutant aPc051310 still exhibits a high productivity. All the results prove that the plasma mutation method could be developed as a convenient and effective tool to breed high-yield strains in the fermentation industry, while expanding the plasm application at the same time. 34-1187/TL |
| ISSN: | 1009-0630 |
| DOI: | 10.1088/1009-0630/14/4/06 |