Analysis of eukaryotic translation in purified and semipurified systems

Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of...

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Bibliographic Details
Published inCurrent protocols in cell biology Vol. Chapter 11; p. Unit 11.9
Main Authors Merrick, W C, Hensold, J O
Format Journal Article
LanguageEnglish
Published United States 01.05.2001
Subjects
Animals
Cell Extracts
Cytological Techniques
Humans
Molecular Biology - methods
Protein Biosynthesis - physiology
Ribosomes - physiology
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Summary:Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.
ISSN:1934-2616
DOI:10.1002/0471143030.cb1109s08