Synthesis and Relaxivities of Oligonucleotides with TEMPO-labeled Sugar Moiety

Abstract 5-Uridin derivative, UUT, having TEMPO radical at the 2′-position of sugar moiety, was prepared and characterized by X-ray crystallography. The single strands, ssUUT, incorporating UUT and the double strands, dsUUT, with complimentary strand were also obtained. Their EPR spectra and relaxiv...

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Published inNucleic Acids Symposium Series Vol. 52; no. 1; pp. 375 - 376
Main Authors Okazaki, Manami, Sato, Yuichiro, Karasawa, Satoru, Koga, Noboru
Format Journal Article
LanguageEnglish
Published England Oxford University Press 2008
Subjects
Carbohydrates - chemistry
Cyclic N-Oxides - chemistry
DNA - chemistry
DNA, Single-Stranded - chemistry
Electron Spin Resonance Spectroscopy
Models, Molecular
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Spin Labels
Uridine - analogs & derivatives
Uridine - chemistry
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Summary:Abstract 5-Uridin derivative, UUT, having TEMPO radical at the 2′-position of sugar moiety, was prepared and characterized by X-ray crystallography. The single strands, ssUUT, incorporating UUT and the double strands, dsUUT, with complimentary strand were also obtained. Their EPR spectra and relaxivities (25 MHz and 0.59 T) in water were measured. The values of τR obtained from the simulation of EPR spectra and the water-proton relaxivities, r1 and r2, increased in order of UUT, ssUUT, and dsUUT.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrn189