Purification of the eukaryotic 20S proteasome

• Published in: Current protocols in protein science Vol. Chapter 21; p. Unit 21.6
• Main Authors: Wilk, S, Chen, W E
• Format: Journal Article
• Language: English
• Published: United States 01.11.2001
• Subjects: Animals; Cattle; Pituitary Gland - enzymology; Proteasome Endopeptidase Complex - isolation & purification; Proteasome Endopeptidase Complex - metabolism

The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell. Combination of the 20S proteasome with a complex of regulatory proteins forms the 26S proteasome, which in turn is responsible for the recognition and degradation of ubiquitin-protein conjugates. As...