Purification of the eukaryotic 20S proteasome

The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell. Combination of the 20S proteasome with a complex of regulatory proteins forms the 26S proteasome, which in turn is responsible for the recognition and degradation of ubiquitin-protein conjugates. As...

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Bibliographic Details
Published inCurrent protocols in protein science Vol. Chapter 21; p. Unit 21.6
Main Authors Wilk, S, Chen, W E
Format Journal Article
LanguageEnglish
Published United States 01.11.2001
Subjects
Animals
Cattle
Pituitary Gland - enzymology
Proteasome Endopeptidase Complex - isolation & purification
Proteasome Endopeptidase Complex - metabolism
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Summary:The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell. Combination of the 20S proteasome with a complex of regulatory proteins forms the 26S proteasome, which in turn is responsible for the recognition and degradation of ubiquitin-protein conjugates. As described in this unit, the constitutive form of the 20S proteasome can be conveniently purified as a stable and homogeneous preparation from bovine pituitaries. A support protocol details an enzyme assay used in evaluating proteasomal activity. The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell.
ISSN:1934-3663
DOI:10.1002/0471140864.ps2106s25