Processing and staining of cell and tissue material for interphase cytogenetics

This unit describes in detail the protocols for preparation of biological specimens (fresh or fixed) for the purposes of establishing in situ hybridization procedures using probes for specific regions of the genome, allowing targeted detection of numerical and structural chromosome aberrations in th...

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Bibliographic Details
Published inCurrent protocols in cytometry Vol. Chapter 8; p. Unit 8.5
Main Authors Hopman, A H, Ramaekers, F C
Format Journal Article
LanguageEnglish
Published United States 01.05.2001
Subjects
Animals
Chromosome Aberrations
Cytogenetics
Cytological Techniques
Genome
Humans
In Situ Hybridization
Interphase
Oligonucleotide Probes - analysis
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Summary:This unit describes in detail the protocols for preparation of biological specimens (fresh or fixed) for the purposes of establishing in situ hybridization procedures using probes for specific regions of the genome, allowing targeted detection of numerical and structural chromosome aberrations in the interphase nucleus. The authors present a series of comprehensive protocols from the simplest to the most complex.
ISSN:1934-9300
DOI:10.1002/0471142956.cy0805s05