Consistency of direct microscopic examination and ELISA in detection of Giardia in stool specimen among children

To investigate the consistency of direct microscopic examination and ELISA for determination of Giadia in stool specimen. Study population consisted of children with any clinical symptoms of Giardia infestation since last two weeks. Fresh stool specimen was collected from each child. The stools spec...

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Published inAsian Pacific journal of tropical disease Vol. 4; pp. S725 - S727
Main Authors Torabi, Zohreh, Niksirat, Ali, Mazloomzadeh, Saeideh, Ahmadiafshar, Akefeh
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.09.2014
Subjects
Constipation
Direct stool exam
ELISA method
Giardia lamblia
ELISA method
Giardia lamblia
Constipation
Direct stool exam
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Summary:To investigate the consistency of direct microscopic examination and ELISA for determination of Giadia in stool specimen. Study population consisted of children with any clinical symptoms of Giardia infestation since last two weeks. Fresh stool specimen was collected from each child. The stools specimens were assessed by two methods of direct microscopic examination and ELISA. The degree of agreement between direct stool exam and ELISA was calculated by Cohen's kappa coefficient. In this study, 124 children with age range 2–12 years were investigated. A total of 64 (61.7%) and 79 (65.7%) of children had Giardia by direct stool exam and ELISA test respectively. There was association between frequency of constipation and Giardia infection (P=0.036). The Cohen's kappa coefficient calculated for degree of agreement between direct stool exam and ELISA showed κ=0.756 (P<0.001). The frequency of Giardia infection in symptomatic children was high and there was high agreement rate between ELISA and direct stool smear.
ISSN:2222-1808
2222-1808
DOI:10.1016/S2222-1808(14)60715-5