ChIP-chip for genome-wide analysis of protein binding in mammalian cells

ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the...

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Bibliographic Details
Published inCurrent protocols in molecular biology (Print) Vol. Chapter 21; p. Unit 21.13
Main Authors Kim, Tae Hoon, Barrera, Leah O, Ren, Bing
Format Journal Article
LanguageEnglish
Published United States 01.07.2007
Subjects
Animals
Cell Line
Chromatin Immunoprecipitation - methods
DNA - isolation & purification
DNA - metabolism
Genomics - methods
Humans
Mammals - genetics
Mammals - metabolism
Oligonucleotide Array Sequence Analysis - methods
Protein Binding
Proteins - isolation & purification
Proteins - metabolism
Proteomics - methods
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Summary:ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. UNIT 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells.
ISSN:1934-3647
DOI:10.1002/0471142727.mb2113s79