First report of natural infection of phlebotomines for Leishmania (Leishmania) chagasi captured in Ponta Porã, on the border between Brazil and Paraguay
Visceral leishmaniasis has been reported in all Brazilian regions and, in Mato Grosso do Sul State, the occurrence of cases has increased significantly. The objective of this study was to identify the natural infection of phlebotomines by Leishmania in Ponta Porã, a Brazilian county bordering Paragu...
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Published in | Asian Pacific journal of tropical disease Vol. 1; no. 4; pp. 253 - 258 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.12.2011
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Subjects | |
Online Access | Get full text |
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Summary: | Visceral leishmaniasis has been reported in all Brazilian regions and, in Mato Grosso do Sul State, the occurrence of cases has increased significantly. The objective of this study was to identify the natural infection of phlebotomines by Leishmania in Ponta Porã, a Brazilian county bordering Paraguay.
By using light CDC and Shannon traps, 185 sandfly females were captured, dissected and arranged in 107 pools subjected to DNA extraction and amplification by Polymerase chain reaction.
From the samples subjected to amplification, the fragment of 120 bp characteristic of
Leishmania sp was observed in one of the groups, which is composed by a female species of
Evandromyia cortelezzii (Brèthes, 1923).
The minimum infection rate calculated for Ponta Porã was 0.54%, and the occurrence of flagellates and the confirmation of natural infection by
Leishmania chagasi pose a serious concern for the transmission of visceral leishmaniasis in the region. |
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ISSN: | 2222-1808 2222-1808 |
DOI: | 10.1016/S2222-1808(11)60060-1 |