Electrochemical aptasensor utilizing pulsed-electrodeposition of MXene film for estradiol detection

• Published in: Journal of the Korean Physical Society Vol. 84; no. 6; pp. 454 - 461
• Main Authors: Min, Seonki, Noh, Daegwon, Oh, Eunsoon
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Physical Society 01.03.2024; Springer Nature B.V
• Subjects: Binding; Chemical sensors; Electrodeposition; Electrodes; Mathematical and Computational Physics; MXenes; Original Paper - Condensed Matter; Particle and Nuclear Physics; Physics; Physics and Astronomy; Sex hormones; Substrates; Theoretical; Electrochemistry; Nanomaterials; Sensors; MXene
• ISSN: 0374-4884; 1976-8524
• DOI: 10.1007/s40042-024-01006-w

Detection of endocrine disrupting chemicals is important for human and animal safety. 17β-estradiol (E2) is a female hormone, which may affect premature puberty. Milk, or certain medicines contain E2 hormone, and various E2 sensors have been developed. We investigated an electrochemical sensor that utilizes aptamers to detect the E2 hormone. For an active layer of the aptasensor, we deposited a Ti 3 C 2 T x MXene film using pulsed-electrodeposition (PED) on an ITO substrate. An XRD pattern of the PED-MXene film indicated that it was preferentially aligned along (002) direction. We compared electrochemical sensing performance of the PED-MXene aptasensor with an aptasensor without MXene. Before we immobilized E2-binding aptamers, the electrode surfaces were modified with carboxyl groups (-COOH) using succinic anhydride. After the surface modification, we immobilized the E2-binding aptamer on the electrode surfaces via EDC/NHS reaction. We observed a significant improvement in the sensitivity utilizing the MXene electrode, and we were able to successfully detect E2 below 1 pM concentration.