Development of novel microsatellite markers for aquaculture important species, milkfish (Chanos chanos Forsskål, 1775) with implications of conservation and management
A total of 20 polymorphic microsatellites were developed and characterized for a potential aquaculture species, Chanos chanos , utilizing the comprehensive whole genome data of C. chanos obtained from the GenBank database (accession number: LR697106). Using the MIcroSAtellite (MISA) tool, potential...
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Published in | Conservation genetics resources Vol. 16; no. 3; pp. 209 - 212 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.09.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A total of 20 polymorphic microsatellites were developed and characterized for a potential aquaculture species,
Chanos chanos
, utilizing the comprehensive whole genome data of
C. chanos
obtained from the GenBank database (accession number: LR697106). Using the MIcroSAtellite (MISA) tool, potential microsatellite motifs were identified in the assembled contigs and unigenes and with the PRIMER 3 input software (Version 4.1.0), new primers were developed. The number of alleles per locus in
C. chanos
varied from 9 to 16, with observed heterozygosities ranging from 0.710 to 0.926, while the expected heterozygosities were within the range of 0.720 to 0.902. Among the 20 microsatellite loci, the majority exhibited PIC values > 0.75. Validation of these microsatellite markers proved that these markers can be used as a significant tool for analysing genetic differentiation as well genetic relationships among
C. chanos
. |
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ISSN: | 1877-7260 1877-7252 1877-7260 |
DOI: | 10.1007/s12686-024-01354-6 |