Detection of infectious laryngotracheitis virus in chickens using a non-radioactive DNA probe

A DNA hybridization assay using a non-radioactive probe has been developed for the detection of infectious laryngotracheitis virus (ILTV) DNA. A 1.4-kilobase pair BamHI fragment of ILTV genomic DNA was cloned and then labeled by one of two methods; nick translation using 32P-dATP or non-radioactive...

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Bibliographic Details
Published inAvian diseases Vol. 35; no. 2; p. 257
Main Authors Keam, L. (Commonwealth Scientific and Industrial Research Organisation, Parkville, Victoria, Australia), York, J.J, Sheppard, M, Fahey, K.J
Format Journal Article
LanguageEnglish
Published United States 01.04.1991
Subjects
ADN
Animals
Antigens, Viral - analysis
AVES DE CORRAL
Chickens
Cloning, Molecular
DIAGNOSTIC
DIAGNOSTICO
DNA Probes
DNA, Viral - analysis
Enzyme-Linked Immunosorbent Assay
Herpesviridae Infections - diagnosis
Herpesviridae Infections - microbiology
Herpesviridae Infections - veterinary
Herpesvirus 1, Gallid - genetics
Herpesvirus 1, Gallid - immunology
Herpesvirus 1, Gallid - isolation & purification
LARINGOTRAQUEITIS
LARYNGOTRACHEITE
Nucleic Acid Hybridization
Poultry Diseases - diagnosis
Poultry Diseases - microbiology
Predictive Value of Tests
RADIACTIVIDAD
RADIOACTIVITE
Specific Pathogen-Free Organisms
Trachea - microbiology
VOLAILLE
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Summary:A DNA hybridization assay using a non-radioactive probe has been developed for the detection of infectious laryngotracheitis virus (ILTV) DNA. A 1.4-kilobase pair BamHI fragment of ILTV genomic DNA was cloned and then labeled by one of two methods; nick translation using 32P-dATP or non-radioactive labeling using a commercially available DNA labeling and detection kit. The non-radioactive DNA labeling method proved to be as sensitive as the radioactive method. Using the non-radioactive probe, ILTV DNA was readily detected in tracheal samples from acutely infected chickens and also from convalescent chickens at a time when viral antigen could no longer be detected by the enzyme-linked immunosorbent assay or the virus could no longer be reisolated. This technique provides a safe and effective means of identifying field outbreaks of ILTV and also may detect latent ILTV infections relatively quickly and inexpensively
Bibliography:L73
9300386
ISSN:0005-2086
1938-4351
DOI:10.2307/1591174