Speciation of Thulium Porphyrin Complexes by Reversed-Phase HPLC

• Published in: Journal of liquid chromatography & related technologies Vol. 26; no. 6; pp. 911 - 923
• Main Authors: Zhang, Jun-Feng, Wang, Hong, Hou, An-Xin, Wang, Chang-Fa, Zhang, Hua-Shan
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.04.2003; Taylor & Francis
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; HPLC; Other chromatographic methods; Porphyrin; Thulium; Cationic complex; Reversed phase chromatography; Chemical analysis; Thulium Complexes; Phase composition; HPLC chromatography; Organic ligand; Visible spectrometry; Metalloporphyrin; Lanthanide Complexes; Mobile phase; Speciation
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-120018892

Six new thulium porphyrin complexes have been separated by high performance liquid chromatography with triethanolamine (TEOLA) and acetic acid (HAc) as additives in the methanol-water mixture. The function of TEOLA and HAc has been described. The whole analysis was completed on a C 18 column in 16 min at a flow rate of 0.5 mL min −1 , with the mobile phase of methanol-water (93:7,v:v) containing 75 mmol L −1 TEOLA and 200 mmol L −1 HAc. The detection limits (S/N = 3) of thulium porphyrins at 420 nm were 1.0, 1.0, 1.0, 1.0, 5.0, and 5.0 ng mL −1 , respectively. Compared with the methanol system, it can attain the equivalent separation with the mobile phase of ethanol-water (90:10,v:v) containing 25 mmol L −1 TEOLA and 200 mmol L −1 HAc pumped at 0.25 mL min −1 .