Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)

Thin-lipped mullet ( Chelon ramada ) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-s...

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Published inBiológia Vol. 79; no. 7; pp. 2151 - 2156
Main Authors Meziane, Khadidja, Guinand, Bruno, Kara, Mohamed Hichem, Chaoui, Lamya
Format Journal Article
LanguageEnglish
Published Cham Springer International Publishing 01.07.2024
Springer Nature B.V
Subjects
Amplification
Aquaculture
Biomedical and Life Sciences
Candidate species
Cell Biology
Chelon ramada
Genetic markers
Life Sciences
Microbiology
Microsatellites
Plant Sciences
Polymerase chain reaction
Short Communication
Stock assessment
Zoology
Microsatellite
Resource sharing
High-throughput sequencing
Mediterranean Sea
Mugilids
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Summary:Thin-lipped mullet ( Chelon ramada ) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.
ISSN:1336-9563
0006-3088
1336-9563
DOI:10.1007/s11756-024-01683-9