The effect of medical ozone on oxidative stress and neuroinflammation in the early stage after experimental status epilepticus

• Published in: Biológia Vol. 76; no. 12; pp. 3875 - 3882
• Main Authors: Cokyaman, Turgay, Oztopuz, Ozlem, Coskun, Ozlem, Buyuk, Basak, Kiraz, Hasan A, Elmas, Sait
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2021; Springer Nature B.V
• Subjects: Animal models; Antioxidants; Apoptosis; Biomedical and Life Sciences; Blood; Brain; Cell Biology; Edema; Eosinophilia; Epilepsy; IL-1β; Inflammation; Injury prevention; Life Sciences; Lithium; Microbiology; mRNA; Neurological complications; Neuroprotection; Original Article; Oxidative stress; Ozone; Peripheral blood; Pilocarpine; Plant Sciences; Seizures; Tumor necrosis factor-α; Zoology; Rats; Neuroprotection; Ozone; Lithium; Pilocarpine; Status epilepticus
• ISSN: 0006-3088; 1336-9563
• DOI: 10.1007/s11756-021-00911-w

Preservation of the brain is important to reducing recurrent seizures and other neurological sequelae after status epilepticus (SE). Medical ozone (MedO 3 ) shows antioxidant, anti-inflammatory and anti-apoptotic properties in the human body. In this study, it was aimed to understand the neuroprotective potential of MedO 3 in the acute period after SE. Mature rats of Wistar Albino were used for the study. Group design O 3  + SE: SE induced after MedO 3 , O 3 : MedO 3 alone was given, SE: SE only induced, SE + O 3 ; MedO 3 given after SE, C: control and S: sham control group. SE was induced with lithium-pilocarpine experimental model and evaluated on the Racine scale. Peripheral blood samples and brain tissue samples were taken before decapitation. Histopathological evaluation of the hippocampus of the rats given medical O 3 before and after SE were studied. The highest peripheral blood oxidative stress index (OSI) was found in SE group. The OSI level in O 3  + SE and SE + O 3 was significantly higher than SE/C/S. Gene expressions of TNF-α and IL-1β mRNA were significantly higher in SE compared to other groups. Histopathologically; eosinophilia, cellular shrinkage and interstitial oedema were detected in the most severe SE and to a lesser extent in O 3  + SE/ SE + O 3 . MedO 3 reduced SE-induced oxidative damage, neuroinflammation and neuronal injury and contributed positively to neuroprotection.