Investigating the endophytic competency and pathogenicity efficacy of Beauveria bassiana isolates against Chilo partellus Swinhoe

• Published in: International journal of tropical insect science Vol. 42; no. 2; pp. 1225 - 1237
• Main Authors: Bancole, Wonroo Bernice Armelle, Laing, Mark Delmege, Yobo, Kwasi Sackey, Togola, Abou, Saeed, Mohamed Baha
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.04.2022; Springer Nature B.V
• Subjects: Beauveria bassiana; Biodiversity; Biological control; Biomedical and Life Sciences; Borers; Cereal crops; Chilo partellus; Colonization; Cultivars; Economic importance; Endophytes; Entomology; Human nutrition; Inoculation; Inoculum; Insecticides; Insects; Larvae; Life Sciences; Livestock; Livestock feeds; Nutrition; Original Research Article; Pathogenicity; Pathogens; Pest control; Pests; Plant tissues; Seed treatments; Sorghum; Sorghum bicolor; Stems; Endophytic; Biocontrol
• ISSN: 1742-7592; 1742-7584; 1742-7592
• DOI: 10.1007/s42690-021-00641-1

Sorghum bicolor L. Moench (Poaceae) is a cereal crop grown worldwide and playing an important role in human nutrition as well as feed for livestock. Lepidopteran stem borers are the most economically important insect pests that affect sorghum production and their management relies on the use of insecticide chemicals that have adverse effects on humans and biodiversity. The objective of this study was to investigate the endophytytic and pathogenic properties of Beauveria bassiana (Balsamo) Vuillemin as environmentally friendly, ecologically sound and sustainable bioncontrol method against Chilo partellus Swinhoe, one of the key stemborer of Sorghum in South Africa. In this study, 20 strains of Beauveria bassiana (Balsamo) Vuillemin were tested on sorghum cultivars Sugargraze AS 18, AS 79, AS 71 and PAN 8816 initially infested with Chilo partellus Swinhoe at 3rd instar larval stage. For the purpose, various strains of B. bassiana were inoculated in sorghum cultivars as foliar spray or seed treatment inoculum. At 30 and 60 days after the inoculation, the roots, stems and leaves of the cultivars were sampled to evaluate the endophytic property of each B. bassiana strain. Moreover, the 3rd instar larvae of C. partellus were fed with harvested stems previously colonized by the five best strains of B. bassiana to test their pathogenicity. Some 3rd instar larvae were fed with untreated stems (check). On both treated and untreated plants, number of dead larva was counted and recorded every seven days for 28 days interval. Results showed good endophytic ability of five B. bassiana strains (Bb3, Bb4, Bb10, Bb21 and Bb35) in the sorghum plants with varying degree of colonization and persistence in plant tissues, regardless the inoculation methods. However, colonization resulted from seed treatments were higher than that from foliar spray. The untreated plants did not show any presence of B. bassiana strains. Significant main effects (mostly P<0.0001) and interaction effects were observed between the application methods, strains, cultivars and date after inoculation. The endophytic strains (B3, B10 and B35) were highly pathogenic, recording up to 80% of larval mortality at 28 days after treatment. Five strains of B. bassiana (Bb3, Bb4, Bb10, Bb21 and Bb35) showed both endophytic colonization in S. bicolor and entomopathogenic activity on C. partellus, and can be used as potential systemic biocontrol agents against the spotted stem borer.