Biochemical studies on influenza viruses I. Comparative analysis of equine 2 virus and virus N genes and gene products

Ribonucleic acid (RNA) from equine 2 and virus N was analyzed by polyacrylamidegel electrophoresis and separate RNA bands isolated. Base sequence homologies with various influenza prototype strains were established and gene functions assigned to particular RNA segments. Recombinant strains obtained...

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Published inVirology (New York, N.Y.) Vol. 79; no. 2; pp. 393 - 404
Main Authors Rohde, Wolfgang, Harms, Etti, Scholtissek, Christoph
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.06.1977
Subjects
Animals
Base Sequence
Electrophoresis, Polyacrylamide Gel
Horses
Influenza A virus - analysis
Influenza A virus - isolation & purification
Nucleic Acid Hybridization
Protein Biosynthesis
Recombination, Genetic
RNA, Viral - analysis
Viral Proteins - analysis
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Summary:Ribonucleic acid (RNA) from equine 2 and virus N was analyzed by polyacrylamidegel electrophoresis and separate RNA bands isolated. Base sequence homologies with various influenza prototype strains were established and gene functions assigned to particular RNA segments. Recombinant strains obtained by double infection of chick embryo cells with the fowl plague virus (FPV) mutant ts 113, temperature-sensitive in neuraminidase, and with equine 2 virus or virus N, respectively, carry the neuraminidase gene of the rescue virus and were characterized biochemically. Evidence is presented that equine 2-specific neuraminidase consists of subunits of different molecular weights.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(77)90366-X