Label-free electrochemical biosensor based on GR5 DNAzyme/Ti3C2Tx Mxenes for Pb2+ detection

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 905; p. 115979
• Main Authors: Liu, Yinyin, Qiu, Ruhan, Zhang, Zhicheng, Chen, Dawei, Gao, Yushi, Liu, Zongping, Li, Haidong, Wang, Chengyin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.01.2022; Elsevier Science Ltd
• Subjects: Adenine; Adsorptivity; Biosensors; Cleavage; Electrochemical biosensor; Environmental monitoring; Glassy carbon; GR5 DNAzyme; Inspection; Label-free; MXenes; Nanomaterials; Nucleic acids; Oxidation; Pb2+ ion; Substrates; Ti3C2Tx Mxenes; Ti3C2Tx Mxenes; Pb2+ ion; Electrochemical biosensor; GR5 DNAzyme; Label-free
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2021.115979

•The interactions of Ti3C2Tx Mxenes toward different DNA molecules were studied.•Pb2+ could enhance the oxidation peak current of GR5 DNAzyme/Ti3C2Tx Mxenes GCE.•Label-free sensor was fabricated based on GR5 DNAzyme/Ti3C2Tx Mxenes GCE.•This sensor was successfully applied to detect Pb2+ in rat and chicken samples. The interactions between nucleic acids and nanomaterials have been often utilized to design the electrochemical biosensor due to easy preparation, adsorptive tunability and high sensitivity. In this study, we have prepared a new electrochemical biosensor for Pb2+ detection based on GR5 DNAzyme/Ti3C2Tx Mxenes modified glassy carbon electrode (GR5 DNAzyme/Ti3C2Tx Mxenes-GCE). GR5 DNAzyme is an RNA-cleavage-type DNAzyme that shows high specificity towards Pb2+. With the presence of Pb2+, the cleavage of GR5 DNAzyme is activated at a ribo-adenine (rA) site in the substrate stranded DNA, leading to the unwinding of double-helix structure. This can enhance the adsorptive property of Ti3C2Tx Mxenes toward GR5 DNAzyme, which favors the ion intercalation into the Ti3C2Tx Mxenes and induces the increase of oxidation peak current. Therefore, we have successfully applied GR5 DNAzyme/Ti3C2Tx Mxenes-GCE to detect Pb2+ with a linear range from 0.5 to 32 nM and a detection limit of 0.1 nM. This electrochemical biosensor is simple, sensitive and selective for Pb2+ determination that it should find some potential applications in food safety inspection and environmental monitoring.