Cloning and functional characterization of auxin receptor TIR1 in Gossypium hirsutum
Transport inhibitor response 1 ( TIR1 ), as an F-box family protein, is a nucleus receptor of auxin in plants. It is essential in the auxin signal transduction pathway and the expression of auxin-responsive genes. As the most important hormone in plants, auxin has a great influence on the ovule and...
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Published in | Acta physiologiae plantarum Vol. 43; no. 3 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.03.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Transport inhibitor response 1 (
TIR1
), as an F-box family protein, is a nucleus receptor of auxin in plants. It is essential in the auxin signal transduction pathway and the expression of auxin-responsive genes. As the most important hormone in plants, auxin has a great influence on the ovule and fiber development of cotton (
Gossypium hirsutum
L.). Studies on the effect of auxin signal transduction pathway on cotton fiber development can fundamentally solve the problem of fiber yield and quality. The cDNA sequence of
G. hirsutum TIR1
was obtained through homologous cloning and rapid-amplification of cDNA ends (RACE). The homology between
GhTIR1
and that in
Arabidopsis thaliana
was 64.8%. To study the functions, subcellular localization, and the specific localization of
GhTIR1
sequence, we constructed the fusion expression vector of
GhTIR1
and its N-terminal or C-terminal deletion mutant genes with
GFP
. By transiently transforming
Arabidopsis
protoplasts and stably transforming
Nicotiana tabacum
BY-2 cell lines,
GhTIR1
was localized in the nucleus through the N-terminal 1–197 amino acid sequence. The expression patterns of
GhTIR1
in different organs and ovule and cotton fiber cells at different developmental stages showed that
GhTIR1
was highly expressed in the initial and elongation stages of cotton fiber development. These results indicate that
GhTIR1
plays an important role in the initiation and elongation of cotton fibers, especially the formation of primary fibers of cotton fiber cells. |
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ISSN: | 0137-5881 1861-1664 |
DOI: | 10.1007/s11738-020-03198-7 |