Synthesis optimization of PEG diblock copolymer-based nanoemulsion of cypermethrin through central composite design and bioefficacy evaluation against fish ectoparasite Argulus bengalensis

• Published in: Chemical papers Vol. 76; no. 11; pp. 6809 - 6820
• Main Authors: Sarkar, Dhruba Jyoti, Bera, Asit Kumar, Baitha, Raju, Das, Basanta Kumar
• Format: Journal Article
• Language: English
• Published: Warsaw Versita 01.11.2022; Springer Nature B.V
• Subjects: Biochemistry; Biotechnology; Block copolymers; Chemical synthesis; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Eggs; Fish; Industrial Chemistry/Chemical Engineering; Materials Science; Mathematical analysis; Medicinal Chemistry; Nanoemulsions; Optimization; Original Paper; Response surface methodology; Zeta potential; Diblock copolymer; Cypermethrin; Nanoformulation
• ISSN: 0366-6352; 1336-9075; 2585-7290
• DOI: 10.1007/s11696-022-02369-9

Nanosizing antiparasitic formulation has tremendous potential to control parasite infection by rectifying drawbacks of conventional formulations. The present study investigates synthesis optimization of cypermethrin nanoemulsion using PEG diblock copolymers through a spontaneous emulsification technique. Synthesis parameters of nanoemulsion were optimized using response surface methodology with central composite design to get nanoemulsion (NEC-1) with lowest particle size (179.8 nm) and highest encapsulation efficiency (90.24%). TEM and DLS revealed the spherical morphology and negative zeta potential (− 42.4–57.2 mV) of the developed nanoemulsion formulations. Bioefficacy of the NEC-1 was studied against fish ectoparasite Argulus bengalensis at embryonic and adult stages. It was observed that treatment of nanoemulsion disrupted the natural developmental stage of A. bengalensis egg at a very low concentration (0.001 ppm). The calculated LC 50 against the adult parasite and LC 50 for prevention of 50% egg hatching was found to be 0.005 and 0.006 ppm, respectively. In the recent scenario of repetitive antiparasitic resistance, the developed nanoemulsion could come out as a potential solution; however, further study is required for its feasibility for use in actual field conditions.