Asymmetrical labeling of giant phospholipid vesicles

The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the a...

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Bibliographic Details
Published inPflügers Archiv Vol. 440; no. 5 Suppl; pp. R51 - R052
Main Authors Gomiscek, G, Arrigler, V, Gros, M, Yupncic, M, Svetina, S
Format Journal Article
LanguageEnglish
Published Germany 2000
Subjects
4-Chloro-7-nitrobenzofurazan - analogs & derivatives
Dithionite - pharmacology
Fluorometry
Membranes, Artificial
Microscopy, Fluorescence
Phosphatidylcholines
Phospholipids - metabolism
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Summary:The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50% decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, fluorimetry as well as fluorescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0031-6768
1432-2013
DOI:10.1007/s004240000003