MOLECULAR CHARACTERIZATION OF SPOROCYSTS AND CERCARIAE (DIGENEA: BUCEPHALIDAE) INFECTING THE EASTERN OYSTER CRASSOSTREA VIRGINICA FROM VIRGINIA

The identity of a larval bucephalid infecting the eastern oyster, Crassostrea virginica (Gmelin, 1791), from a tidal river in Virginia is investigated using ribosomal deoxyribonucleic acid (rDNA). The internal transcribed spacer (ITS1, 5.8S, ITS2) region and a portion of the 28S rDNA were isolated f...

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Published inThe Journal of parasitology Vol. 109; no. 3; pp. 259 - 263
Main Authors Curran, Stephen S, Gonzales, Robert D, Bullard, Stephen A
Format Journal Article
LanguageEnglish
Published United States Allen Press Inc 01.05.2023
Subjects
Animals
Cercaria
Cercaria - genetics
Crassostrea
Crassostrea virginica
Deoxyribonucleic acid
DNA
DNA, Ribosomal
Fish
Fourier transforms
Laboratories
Larva
Nucleotide sequence
Oocysts
Oysters
Parasitology
Prosorhynchoides
Ribosomal DNA
Sporocysts
Tidal rivers
Tidal waterways
Trematoda - genetics
Virginia
Virginia
Prosorhynchoides
parasite
Crassostrea virginica
ITS2
28S rDNA
Bucephalidae
ITS1
Rhipidocotyle
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Summary:The identity of a larval bucephalid infecting the eastern oyster, Crassostrea virginica (Gmelin, 1791), from a tidal river in Virginia is investigated using ribosomal deoxyribonucleic acid (rDNA). The internal transcribed spacer (ITS1, 5.8S, ITS2) region and a portion of the 28S rDNA were isolated from genomic DNA from sporocysts containing cercariae and compared with sequences from GenBank and from our previous collections of possibly related bucephalids. The studied larval bucephalid was 100% identical at the ITS1, 5.8S, and partial 28S rDNA sequences with Prosorhynchoides paralichthydis (Corkum, 1961) Curran and Overstreet, 2009; however, it differed from P. paralichthydis by 6 bases plus 3 deletions in the ITS2 region. This level of variation at the ITS2 region has been demonstrated among some Indo-Pacific species of Prosorhynchoides Dollfus, 1929, suggesting that the larval bucephalid represents an unidentified or innominate species of Prosorhynchoides that is closely related to P. paralichthydis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0022-3395
1937-2345
DOI:10.1645/22-100