Characterization of CLCN5 and Expression Profile under Low-Salinity Stress in Takifugu rubripes

ABSTRACT Voltage-gated chloride channel 5 (CLCN5) transports hydrogen ions into the nucleolus and removes chloride ions, which helps the cell to maintain its ph value and osmotic pressure. To reveal the role of CLCN5 under low-salinity stress, a cDNA sequence of CLCN5 (TrCLCN5) was cloned from Takif...

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Published inPakistan journal of zoology Vol. 54; no. 2; p. 835
Main Authors Ye, Lin, Jiang, Jie-Lan, Xu, Jia, Liu, Rui-Ting, Tian, Yin-Qiu, Zang, Qing-Qing, Cao, Jia-Yi, Mao, Ming-Guang
Format Journal Article
LanguageEnglish
Published Lahore Knowledge Bylanes 30.04.2022
AsiaNet Pakistan (Pvt) Ltd
Subjects
Amino acid sequence
Amino acids
Biotechnology
Chloride
Chloride channels
Chloride ions
Chlorides
Cloning
Complementary DNA
Domains
Experiments
Genetic aspects
Gills
Homology
Hydrogen ions
Intestine
Ion channels
Kidneys
Nucleoli
Osmosis
Osmotic pressure
Phylogenetics
Physiology
Polymerase chain reaction
Proteins
Reverse transcription
Salinity
Salinity effects
Signal transduction
Stress
Stress (Physiology)
Takifugu rubripes
China
United States > US
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Summary:ABSTRACT Voltage-gated chloride channel 5 (CLCN5) transports hydrogen ions into the nucleolus and removes chloride ions, which helps the cell to maintain its ph value and osmotic pressure. To reveal the role of CLCN5 under low-salinity stress, a cDNA sequence of CLCN5 (TrCLCN5) was cloned from Takifugu rubripes using the reverse transcription PCR (RT-PCR) technique. The results showed that TrCLCN5 cDNA contained a complete open reading frame of 2505 bp continuously encoding 834 amino acid residues. Sequence alignments revealed that the TrCLCN5 cDNA is highly homologous (96.2% and 96.1%) with that of Takifugu bimaculatus and Takifugu flavidus. SignalP and TMhMM analysis showed that TrCLCN5-deduced protein was a type III transmembrane protein and lacked a typical signal peptide. Conserved Domain analysis revealed that there was a VoltageCLC domain and two CBS domains located in the TrCLCN5 deduced protein. qPCR analysis showed that TrCLCN5 was highly expressed in the intestine, kidney and liver, and up-regulated in gills under low-salinity stress in 3h to 6h, which indicates that TrCLCN5 may play an important role in the responses to acute low-salinity stress and would be useful in further understanding low-salinity adaption in T. rubripes.
ISSN:0030-9923
0030-9923
DOI:10.17582/journal.pjz/20200828090859