Detection of anaerobic sulfate-reducing bacteria in oxygen-containing upper water layers of the Black and Baltic Seas

• Published in: Moscow University biological sciences bulletin Vol. 70; no. 4; pp. 184 - 188
• Main Authors: Bryukhanov, A. L, Korneeva, V. A, Pimenov, N. V
• Format: Journal Article
• Language: English
• Published: New York Allerton Press 01.10.2015
• Subjects: bacterial communities; basins; Biochemistry; Biomedical and Life Sciences; Cell Biology; Desulfobacter; Desulfosporosinus; Desulfotomaculum; Desulfovibrio; fluorescence in situ hybridization; Life Sciences; Microbiology; phylogeny; Plant Sciences; polymerase chain reaction; ribosomal RNA; seawater; sulfate-reducing bacteria; Zoology; Black Sea; Baltic Sea; fluorescent in situ hybridization; sulfate-reducing bacteria; nested PCR; Black Sea; meromictic basin; Gdansk Deep of the Baltic Sea
• ISSN: 0096-3925; 1934-791X
• DOI: 10.3103/S0096392515040057

Fluorescent in situ hybridization (FISH) and PCR were used for analysis of phylogenetic structure of anaerobic sulfate-reducing bacterial communities in oxygen-containing upper water layers of meromictic basins: the Black Sea and the Gdansk Deep of the Baltic Sea. In the Black Sea (continental slope at depths 30–70 m), cells of sulfate-reducing bacteria (SRB) hybridizing with 16S rRNA-specific FISH-probes for Desulfotomaculum, Desulfobacter, and Desulfovibrio genera were revealed, whereas Desulfomicrobium-related bacteria were prevalent in the chemocline zone at a 150-m depth. Besides Desulfotomaculum (SRB subgroup 1), Desulfobacter (SRB subgroup 4), and Desulfovibrio-Desulfomicrobium (SRB subgroup 6), nested PCR with the use of 16S rRNA gene-specific primers detected the presence of Desulfococcus–Desulfonema–Desulfosarcina (SRB subgroup 5) in the oxygen-containing water column of the Black and Baltic seas. Active enrichment SRB culture that contained bacterium Desulfosporosinus sp. as a major component was obtained from the Black Sea water sample collected at a 70-m depth.