Molecular characterization of Iranian dromedaries using microsatellite markers

• Published in: Acta agronomica (Palmira) Vol. 69; no. 4; pp. 321 - 330
• Main Authors: Mohammadabadi, Mohammadreza, Ghasemi Meymandi, Mehrdad, Montazeri, Mahdieh, Afanasenko, Volodymyr, Kalashnyk, Oleksandr
• Format: Journal Article
• Language: English
• Published: Bogota Universidad Nacional de Colombia 25.11.2021
• Subjects: AGRICULTURE, MULTIDISCIPLINARY; Alleles; Biomarkers; Breeding; Camelus dromedarius; Conservation; Deoxyribonucleic acid; Differentiation; DNA; Genetic analysis; Genetic diversity; Genetic markers; Heterozygosity; Inbreeding; Indigenous animals; Loci; Microsatellites; Population genetics; Population studies; Populations; Relativity; Microsatellites; Poblaciones de dromedarios iraníes; Iranian dromedary populations; Diversidad genética; Population genetic structure; Estructura genética de poblaciones; Genetic diversity; Microsatélites
• ISSN: 0120-2812; 2323-0118
• DOI: 10.15446/acag.v69n4.64487

Considering the importance of maintaining the genetic diversity in native animals, this study conducted to analyse genetic diversity in dromedary populations in the north of Kerman province, Iran, using eight autosomal microsatellite markers. Eighty-one blood samples were collected from five different populations and DNA was extracted. The highest and the lowest allele number and effective alleles were shown in YWLL08 (21 and 4) and VOLP32 (14.97 and 3.11), respectively. The expected heterozygosity varied from 0.778 in Sahra-e Jahad population to 0.847 in Nogh population. The test for Hardy-Weinberg equilibrium showed significant deviations in most loci. The mean multilocus FST value (0.057) suggested that differentiation is moderate between populations. From total genetic diversity, only 6% were due to differentiation among populations, while the remaining 94% corresponded to differences among individuals within each population. The results of the current study indicated that the Camelus dromedarius populations in the north of Kerman province have a relativity high genetic variation and the data could be useful for designing the breeding strategies and conservation. The degree of variability demonstrated implies that studied populations are rich reservoirs of genetic diversity that must be preserved. A future direction to our study can be studying all of the Iranian Camelus dromedarius populations to better evaluate the level of inbreeding and establish the appropriate conservation strategies aimed to avoid losses of genetic diversity.