Application of atmospheric pressure chemical ionisation mass spectrometry in the analysis of barbiturates by high-speed analytical countercurrent chromatography
Four barbiturates (barbital, allobarbital, phenobarbital and butalbital) were analysed using high‐speed analytical countercurrent chromatography (HSACCC) and high‐performance liquid chromatography (HPLC) interfaced with mass spectrometry, using negative mode atmospheric pressure chemical ionisation...
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Published in | Rapid communications in mass spectrometry Vol. 17; no. 14; pp. 1565 - 1572 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
30.07.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Four barbiturates (barbital, allobarbital, phenobarbital and butalbital) were analysed using high‐speed analytical countercurrent chromatography (HSACCC) and high‐performance liquid chromatography (HPLC) interfaced with mass spectrometry, using negative mode atmospheric pressure chemical ionisation (APCI). The polar biphasic solvent system of butyronitrile/acetonitrile/water (1:1:1) was used, in the upper‐stationary, lower‐mobile mode of operation, at a flow rate of 1 mL/min and a rotational speed of 1200 rpm, equating to an applied ‘g’‐field of 177 g. The fractional stationary phase retention (SF) was 0.58. Representative mass spectral data are presented from the HPLC and the HSACCC analyses. Structural information was obtained using source‐induced fragmentation at increased source block voltages. The effect of increasing g‐field on chromatographic resolution is illustrated using the binary base system of butyronitrile/water (1:1), under electrospray ionisation. Copyright © 2003 John Wiley & Sons, Ltd. |
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Bibliography: | istex:B2E6E075BF96FB46F88783667FA0B2FE20F43E02 ArticleID:RCM1086 ark:/67375/WNG-35BWFC6F-8 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0951-4198 1097-0231 |
DOI: | 10.1002/rcm.1086 |