Phytochemical Constituents of Capsella bursa-pastoris and Their Anti-inflammatory Activity

• Published in: Natural product sciences Vol. 24; no. 2; pp. 132 - 138
• Main Authors: Cha, Joon Min, Kim, Dong Hyun, Lee, Tae Hyun, Subedi, Lalita, Kim, Sun Yeou, Lee, Kang Ro
• Format: Journal Article
• Language: English
• Published: 한국생약학회 2018
• Subjects: 약학; Capsella bursa-pastoris; Cruciferae; Sulfur compound
• ISSN: 1226-3907; 2288-9027
• DOI: 10.20307/nps.2018.24.2.132

Phytochemical investigation of 80% MeOH extract of the aerial parts of Capsella bursa-pastoris yielded fourteen compounds (1 - 14). The structures of the compounds were elucidated by spectroscopic methods to be methyl-1-thio-b-D-glucopyranosyl disulfide (1), 10-methylsulphinyl-decanenitrile (2), 11-methyl-sulphinyl-undecanenitrile (3), 1-O-(lauroyl)glycerol (4), phytene-1, 2-diol (5), (3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one (6), loliolide (7), b-sitosterol (8), 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-1-propanone (9), 1-feruloyl-b-D-glucopyranoside (10), pinoresinol-4'-O-b-D-glucopyranoside (11), luteolin (12), quercetin-3-O-b-D-glucopyranoside (13), and luteolin 6-C-b-glucopyranoside (14). Although compound 1 was reported as synthetic compound, 1 was first isolated from natural source. NMR spectral data assignments of 1, 2 and 3 were reported for the first time, and compounds 1 - 14 were for the first time reported from this plant source. The anti-inflammatory effects of 1 - 14 were evaluated in lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cells. Compounds 12 exhibited strong inhibitory effects on nitric oxide production in LPS-activated BV-2 cells with IC50 values of 9.70 mM. KCI Citation Count: 1