Molecular diagnostics of potato infections with PVY and PLRV by immunochromatography

An immunochromatography test system has been developed for molecular diagnostics of the potato virus Y and PLRV infection. To increase a low yield of PLRV and raise antibodies against the PLRV antigen, chimerical virus was constructed comprising the PLRV coat protein and recombinant RNA of a tobamov...

Full description

Saved in:
Bibliographic Details
Published inMoscow University biological sciences bulletin Vol. 71; no. 1; pp. 39 - 44
Main Authors Kondakova, O. A, K. O. Butenko, E. V. Skurat, Yu. F. Drygin
Format Journal Article
LanguageEnglish
Published New York Allerton Press 2016
Subjects
antibodies
antigens
Biochemistry
Biomedical and Life Sciences
Cell Biology
coat proteins
diagnostic techniques
DNA
genes
immunoaffinity chromatography
Life Sciences
Plant Sciences
Potato virus Y
potatoes
recombinant proteins
RNA
Virology
viruses
Zoology
immunochromatography
molecular diagnostics
infection
potato virus Y
potato leafroll virus
chimerical PLRV
Online AccessGet full text

Cover

Abstract An immunochromatography test system has been developed for molecular diagnostics of the potato virus Y and PLRV infection. To increase a low yield of PLRV and raise antibodies against the PLRV antigen, chimerical virus was constructed comprising the PLRV coat protein and recombinant RNA of a tobamovirus, in which capsid protein gene was replaced by the PLRV coat protein gene. Binary vector containing the DNA copy of the recombinant RNA was infectious, and yield of the chimerical virus increased up to 800 times in comparison with the wild type PLRV. On the basis of experience in the development of the diagnostics of viral and viroid infections, rational tactics are proposed for the mass laboratory and field diagnosis of viral infections on the molecular level.
AbstractList An immunochromatography test system has been developed for molecular diagnostics of the potato virus Y and PLRV infection. To increase a low yield of PLRV and raise antibodies against the PLRV antigen, chimerical virus was constructed comprising the PLRV coat protein and recombinant RNA of a tobamovirus, in which capsid protein gene was replaced by the PLRV coat protein gene. Binary vector containing the DNA copy of the recombinant RNA was infectious, and yield of the chimerical virus increased up to 800 times in comparison with the wild type PLRV. On the basis of experience in the development of the diagnostics of viral and viroid infections, rational tactics are proposed for the mass laboratory and field diagnosis of viral infections on the molecular level.
Author Yu. F. Drygin
E. V. Skurat
K. O. Butenko
Kondakova, O. A
Author_xml – sequence: 1
  fullname: Kondakova, O. A
– sequence: 2
  fullname: K. O. Butenko
– sequence: 3
  fullname: E. V. Skurat
– sequence: 4
  fullname: Yu. F. Drygin
BookMark eNp9kF1LwzAUhoNMcJv-AK_MH6ieJE3bXMrwCyoON4delTRNuow1GUmH7N_bMe8Er87F8z4vh3eCRs47jdA1gVtGgN0tAETGBOUkAwKQkjM0JoKlSS7I5wiNjzg58gs0iXEDwLlgYoyWr36r1X4rA26sbJ2PvVURe4N3vpe9x9YZrXrrXcTftl_j-eoLS9fgefm-wvUB267bO6_WwXdDvA1ytz5conMjt1Ff_d4pWjw-LGfPSfn29DK7LxNFcyCJaRrKmZaKpwWpBdNGGwGsBpoZIlmqDSm0IjmIos6MqQtO60wXDVcgFWVTRE6tKvgYgzbVLthOhkNFoDqOUv0ZZXDoyYlD1rU6VBu_D2548l_p5iQZ6SvZBhurjwWFgQMRPKU5-wErVG_2
CitedBy_id crossref_primary_10_1080_09540105_2017_1401044
crossref_primary_10_1007_s12033_017_0035_6
Cites_doi 10.1016/j.trac.2013.11.007
10.1134/S0006297909090065
10.1007/s002160051558
10.1007/978-1-59745-240-3
10.1099/0022-1317-44-1-153
10.1007/978-1-60327-569-9_12
10.1007/s00253-011-3522-x
ContentType Journal Article
Copyright Allerton Press, Inc. 2016
Copyright_xml – notice: Allerton Press, Inc. 2016
DBID FBQ
AAYXX
CITATION
DOI 10.3103/S0096392516010041
DatabaseName AGRIS
CrossRef
DatabaseTitle CrossRef
DatabaseTitleList

Database_xml – sequence: 1
  dbid: FBQ
  name: AGRIS
  url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN
  sourceTypes: Publisher
DeliveryMethod fulltext_linktorsrc
Discipline Biology
Zoology
EISSN 1934-791X
EndPage 44
ExternalDocumentID 10_3103_S0096392516010041
US201600195427
GroupedDBID -56
-5G
-BR
-EM
-Y2
-~C
.86
.VR
06C
06D
0R~
0VY
123
1N0
29M
29~
2J2
2JN
2JY
2KG
2KM
2LR
2VQ
2~H
30V
4.4
408
40D
40E
5VS
67N
6NX
8TC
95-
95.
95~
96X
AAAVM
AABHQ
AAFGU
AAHNG
AAIAL
AAJKR
AANXM
AANZL
AARHV
AARTL
AATNV
AATVU
AAUYE
AAWCG
AAYFA
AAYIU
AAYQN
AAYTO
ABDZT
ABECU
ABFGW
ABFTV
ABHQN
ABJNI
ABJOX
ABKAS
ABKCH
ABMNI
ABMQK
ABNWP
ABQBU
ABSXP
ABTEG
ABTHY
ABTKH
ABTMW
ABULA
ABWNU
ABXPI
ACBMV
ACBRV
ACBYP
ACCUX
ACGFS
ACHSB
ACHXU
ACIGE
ACIPQ
ACKNC
ACMDZ
ACMLO
ACOKC
ACOMO
ACPRK
ACSNA
ACTTH
ACVWB
ACWMK
ADHHG
ADHIR
ADINQ
ADKNI
ADMDM
ADOXG
ADRFC
ADURQ
ADYFF
ADZKW
AEBTG
AEFTE
AEGAL
AEGNC
AEJHL
AEJRE
AEOHA
AEPYU
AESTI
AETLH
AEVLU
AEVTX
AEXYK
AFGCZ
AFLOW
AFNRJ
AFQWF
AFWTZ
AFZKB
AGAYW
AGDGC
AGGBP
AGJBK
AGMZJ
AGQMX
AGWIL
AGWZB
AGYKE
AHAVH
AHBYD
AHKAY
AHSBF
AIAKS
AIIXL
AILAN
AIMYW
AITGF
AJBLW
AJDOV
AJRNO
AKQUC
ALMA_UNASSIGNED_HOLDINGS
ALWAN
AMKLP
AMXSW
AMYLF
AMYQR
AOCGG
ARMRJ
AXYYD
B-.
BA0
BDATZ
BGNMA
CAG
COF
CS3
DDRTE
DNIVK
DPUIP
DU5
EBLON
EBS
EIOEI
EJD
EN4
ESBYG
FBQ
FERAY
FFXSO
FIGPU
FINBP
FNLPD
FRRFC
FSGXE
FWDCC
G-Y
G-Z
GGCAI
GGRSB
GJIRD
GNWQR
GQ6
GQ7
HF~
HG6
HLICF
HMJXF
HRMNR
HZ~
IJ-
IKXTQ
IWAJR
I~X
I~Z
J-C
JBSCW
JZLTJ
KOV
KPH
LLZTM
M4Y
MA-
NPVJJ
NQJWS
NU0
O9-
O93
O9I
O9J
PF0
PT4
QOR
QOS
R89
R9I
RNS
ROL
RPX
RSV
S16
S1Z
S27
S3A
S3B
SAP
SBL
SDH
SHX
SNE
SNPRN
SNX
SOHCF
SOJ
SPISZ
SRMVM
SSLCW
STPWE
SZN
T13
TSG
TUC
U2A
U9L
UG4
UNUBA
UOJIU
UTJUX
UZXMN
VC2
VFIZW
W48
WK8
XU3
YLTOR
ZMTXR
ZOVNA
~A9
AAPBV
AACDK
AAHBH
AAJBT
AASML
AAYXX
ABAKF
ACAOD
ACDTI
ACZOJ
AEFQL
AEMSY
AFBBN
AGRTI
AIGIU
CITATION
H13
SJYHP
ID FETCH-LOGICAL-c2701-fdd253eac5481b93efef903b026f1a34ef18ec17098b6ffb852b6e8d5c0ac23
IEDL.DBID AGYKE
ISSN 0096-3925
IngestDate Thu Sep 12 17:00:53 EDT 2024
Sat Dec 16 11:59:36 EST 2023
Wed Dec 27 19:13:11 EST 2023
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords immunochromatography
molecular diagnostics
infection
potato virus Y
potato leafroll virus
chimerical PLRV
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c2701-fdd253eac5481b93efef903b026f1a34ef18ec17098b6ffb852b6e8d5c0ac23
Notes http://dx.doi.org/10.3103/S0096392516010041
OpenAccessLink https://vestnik-bio-msu.elpub.ru/jour/article/download/297/290
PageCount 6
ParticipantIDs crossref_primary_10_3103_S0096392516010041
springer_journals_10_3103_S0096392516010041
fao_agris_US201600195427
PublicationCentury 2000
PublicationDate 2016
1-2016
2016-1-00
PublicationDateYYYYMMDD 2016-01-01
PublicationDate_xml – year: 2016
  text: 2016
PublicationDecade 2010
PublicationPlace New York
PublicationPlace_xml – name: New York
PublicationTitle Moscow University biological sciences bulletin
PublicationTitleAbbrev Moscow Univ. Biol.Sci. Bull
PublicationYear 2016
Publisher Allerton Press
Publisher_xml – name: Allerton Press
References Drygin (CR1) 2014
Drygin, Blintsov, Grigorenko, Andreeva, Osipov, Varitzev, Uskov, Kravchenko, Atabekov (CR12) 2012; 93
Drygin, Blintsov, Osipov, Grigorenko, Andreeva, Uskov, Varitsev, Anisimov, Novikov, Atabekov (CR11) 2009; 74
Butenko, Skurat, Kondakova, Varitsev, Gavryushina, Drygin, Atabekov (CR16) 2014; 1
Dzantiev, Byzova, Urusov, Zherdev (CR10) 2014; 55
Drygin, Kondakova, Chirkov, Zinovkin, Kiseleva, Atabekov (CR4) 2009
Kondakova, Drygin (CR3) 1999; 4
Wong, Tse (CR9) 2009
Drygin, Chirkov, Kondakova, Zinovkin, Ivanov, Blintsov, Gavryushina, Zherdev, Byzova, Dzantiev, Atabekov, Haverkort, Anisimov (CR2) 2007
Egorov, Osipov, Dzantiev, Gavrilova (CR13) 1991
Posthuma-Trumpie, Korf, van Amerongen (CR7) 2008; 393
Takanami, Kubo (CR5) 1979; 44
Drygin, Skurat, Kondakova, Atabekov, Butenko (CR14) 2015
Weller (CR6) 2000; 366
Zhang, Guo, Wang, Rasooly, Herold (CR8) 2009
Drygin (CR15) 2012
M.G. Weller (4287_CR6) 2000; 366
O.A. Kondakova (4287_CR3) 1999; 4
Yu.F. Drygin (4287_CR15) 2012
K.O. Butenko (4287_CR16) 2014; 1
G. Zhang (4287_CR8) 2009
Yu.F. Drygin (4287_CR12) 2012; 93
Yu.F. Drygin (4287_CR1) 2014
Yu.F. Drygin (4287_CR4) 2009
B.B. Dzantiev (4287_CR10) 2014; 55
Yu.F. Drygin (4287_CR14) 2015
T. Posthuma-Trumpie (4287_CR7) 2008; 393
Y.F. Drygin (4287_CR11) 2009; 74
Y. Takanami (4287_CR5) 1979; 44
R. Wong (4287_CR9) 2009
Yu.F. Drygin (4287_CR2) 2007
A.M. Egorov (4287_CR13) 1991
References_xml – volume: 55
  start-page: 81
  year: 2014
  end-page: 93
  ident: CR10
  article-title: Immunochromatographic methods in food analysis
  publication-title: Trends Anal. Chem.
  doi: 10.1016/j.trac.2013.11.007
  contributor:
    fullname: Zherdev
– start-page: 274
  year: 2007
  end-page: 285
  ident: CR2
  article-title: High-sensitive technologies for molecular diagnostics of potato virus and viroid infections
  publication-title: Potato Production and Innovative Technologies
  contributor:
    fullname: Anisimov
– year: 1991
  ident: CR13
  publication-title: Teoriya i praktika immunofermentnogo analiza
  contributor:
    fullname: Gavrilova
– volume: 74
  start-page: 986
  issue: 9
  year: 2009
  end-page: 993
  ident: CR11
  article-title: Highsensitivity express immunochromatographic method for detection of plant infection by tobacco mosaic virus
  publication-title: Biochemistry (Moscow)
  doi: 10.1134/S0006297909090065
  contributor:
    fullname: Atabekov
– volume: 4
  start-page: 83
  year: 1999
  end-page: 90
  ident: CR3
  article-title: The diagnostics of potato viroid disease with (dien)Pt-DNA probe
  publication-title: Biotekhnol.
  contributor:
    fullname: Drygin
– volume: 393
  start-page: 569
  issue: 2
  year: 2008
  end-page: 582
  ident: CR7
  article-title: Lateral flow (immuno) assay: its strengths
  publication-title: weaknesses, opportunities and threat. A literature survey, Anal. Bioanal. Chem.
  contributor:
    fullname: van Amerongen
– volume: 1
  start-page: 35
  issue: 1
  year: 2014
  end-page: 36
  ident: CR16
  article-title: Field method for diagnosis of potato leafroll virus with test stripes with chimeric virus antibodies
  publication-title: Zashchita Kartofelya
  contributor:
    fullname: Atabekov
– year: 2014
  ident: CR1
  article-title: Detection of the target sequence of nucleotides and target antigene
  publication-title: 8-ya Vserossiiskaya nauchno-prakticheskaya konferentsiya “Molekulyarnaya diagnostika 2014"
  contributor:
    fullname: Drygin
– volume: 366
  start-page: 635
  issue: 6
  year: 2000
  end-page: 645
  ident: CR6
  article-title: Immunochromatographic techniques-a critical review
  publication-title: Fresenius’ J. Anal. Chem.
  doi: 10.1007/s002160051558
  contributor:
    fullname: Weller
– year: 2015
  ident: CR14
  publication-title: Russian Patent 2555534
  contributor:
    fullname: Butenko
– year: 2009
  ident: CR4
  publication-title: Patent WO/2009/123494
  contributor:
    fullname: Atabekov
– year: 2009
  ident: CR9
  publication-title: Lateral Flow Immunoassay
  doi: 10.1007/978-1-59745-240-3
  contributor:
    fullname: Tse
– start-page: 10
  year: 2012
  end-page: 11
  ident: CR15
  publication-title: The strategy and tactics for molecular diagnosis of potato infections in practice, Vtoroe nauchno-prakticheskoe soveshchanie “Geneticheskie i agrotehnologicheskie resursy povysheniya kachestva prodovol’stvennogo i tehnicheskogo kartofelya”
  contributor:
    fullname: Drygin
– volume: 44
  start-page: 153
  issue: 1
  year: 1979
  end-page: 159
  ident: CR5
  article-title: Enzyme-assisted purification of two phloem-limited plant viruses: tobacco necrotic dwarf and potato leafroll
  publication-title: J. Gen. Virol.
  doi: 10.1099/0022-1317-44-1-153
  contributor:
    fullname: Kubo
– start-page: 169
  year: 2009
  end-page: 183
  ident: CR8
  article-title: Immunochromatographic lateral f low strip tests
  publication-title: Biosensors and Biodetection. Methods and Protocols
  doi: 10.1007/978-1-60327-569-9_12
  contributor:
    fullname: Herold
– volume: 93
  start-page: 179
  issue: 1
  year: 2012
  end-page: 189
  ident: CR12
  article-title: High sensitive field test lateral flow immunodiagnostics of PVX infection
  publication-title: Appl. Microbiol. Biotechnol.
  doi: 10.1007/s00253-011-3522-x
  contributor:
    fullname: Atabekov
– start-page: 10
  volume-title: The strategy and tactics for molecular diagnosis of potato infections in practice, Vtoroe nauchno-prakticheskoe soveshchanie “Geneticheskie i agrotehnologicheskie resursy povysheniya kachestva prodovol’stvennogo i tehnicheskogo kartofelya”
  year: 2012
  ident: 4287_CR15
  contributor:
    fullname: Yu.F. Drygin
– volume: 74
  start-page: 986
  issue: 9
  year: 2009
  ident: 4287_CR11
  publication-title: Biochemistry (Moscow)
  doi: 10.1134/S0006297909090065
  contributor:
    fullname: Y.F. Drygin
– volume: 44
  start-page: 153
  issue: 1
  year: 1979
  ident: 4287_CR5
  publication-title: J. Gen. Virol.
  doi: 10.1099/0022-1317-44-1-153
  contributor:
    fullname: Y. Takanami
– start-page: 169
  volume-title: Biosensors and Biodetection. Methods and Protocols
  year: 2009
  ident: 4287_CR8
  doi: 10.1007/978-1-60327-569-9_12
  contributor:
    fullname: G. Zhang
– volume: 366
  start-page: 635
  issue: 6
  year: 2000
  ident: 4287_CR6
  publication-title: Fresenius’ J. Anal. Chem.
  doi: 10.1007/s002160051558
  contributor:
    fullname: M.G. Weller
– volume: 55
  start-page: 81
  year: 2014
  ident: 4287_CR10
  publication-title: Trends Anal. Chem.
  doi: 10.1016/j.trac.2013.11.007
  contributor:
    fullname: B.B. Dzantiev
– volume-title: Russian Patent 2555534
  year: 2015
  ident: 4287_CR14
  contributor:
    fullname: Yu.F. Drygin
– volume-title: Patent WO/2009/123494
  year: 2009
  ident: 4287_CR4
  contributor:
    fullname: Yu.F. Drygin
– volume: 4
  start-page: 83
  year: 1999
  ident: 4287_CR3
  publication-title: Biotekhnol.
  contributor:
    fullname: O.A. Kondakova
– volume: 393
  start-page: 569
  issue: 2
  year: 2008
  ident: 4287_CR7
  publication-title: weaknesses, opportunities and threat. A literature survey, Anal. Bioanal. Chem.
  contributor:
    fullname: T. Posthuma-Trumpie
– volume: 93
  start-page: 179
  issue: 1
  year: 2012
  ident: 4287_CR12
  publication-title: Appl. Microbiol. Biotechnol.
  doi: 10.1007/s00253-011-3522-x
  contributor:
    fullname: Yu.F. Drygin
– volume-title: 8-ya Vserossiiskaya nauchno-prakticheskaya konferentsiya “Molekulyarnaya diagnostika 2014"
  year: 2014
  ident: 4287_CR1
  contributor:
    fullname: Yu.F. Drygin
– volume-title: Teoriya i praktika immunofermentnogo analiza
  year: 1991
  ident: 4287_CR13
  contributor:
    fullname: A.M. Egorov
– volume-title: Lateral Flow Immunoassay
  year: 2009
  ident: 4287_CR9
  doi: 10.1007/978-1-59745-240-3
  contributor:
    fullname: R. Wong
– volume: 1
  start-page: 35
  issue: 1
  year: 2014
  ident: 4287_CR16
  publication-title: Zashchita Kartofelya
  contributor:
    fullname: K.O. Butenko
– start-page: 274
  volume-title: Potato Production and Innovative Technologies
  year: 2007
  ident: 4287_CR2
  contributor:
    fullname: Yu.F. Drygin
SSID ssj0055939
Score 2.0143585
Snippet An immunochromatography test system has been developed for molecular diagnostics of the potato virus Y and PLRV infection. To increase a low yield of PLRV and...
SourceID crossref
springer
fao
SourceType Aggregation Database
Publisher
StartPage 39
SubjectTerms antibodies
antigens
Biochemistry
Biomedical and Life Sciences
Cell Biology
coat proteins
diagnostic techniques
DNA
genes
immunoaffinity chromatography
Life Sciences
Plant Sciences
Potato virus Y
potatoes
recombinant proteins
RNA
Virology
viruses
Zoology
Title Molecular diagnostics of potato infections with PVY and PLRV by immunochromatography
URI https://link.springer.com/article/10.3103/S0096392516010041
Volume 71
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3JTsMwELW6CIkLO2pZKh84gVLFdtZjhVoqoKiii1ouUezYgColVZdD-XrGWZBQ4dBTfEgcZTwZv5nxvEHoRtd6Sp9IQwgZGZZS3PCIKw1wFSLpRUKyUMc7ei9Od2Q9TuxJCdGf0EU8axYZydRQp26lyXRJL-iKD9uxdiFMXatetXVX6gqqth6mT-3C_gJEZhno9R1DP5DlMv-e5NduVFZhspUQTfeZzmFW-7dM6Qn18ZJZc73iTfG1Td64wyccoYMcduJWpifHqCTjE7SXNaLcwOgtSUenaNgr-uXiKDuFp3mccaLwPAFcmuDi9Fa8xDqGi_vjKQ7jCPefX8eYb_CnLjhJxMciASyc82GfoUGnPbzvGnnnBUNQ1ySGiiJqM7DJ4M8Q7jOppPJNxsFhUyRkllTEk4K4pu9xB9bXsyl3YGltYYaCsnNUiZNY1hD2uXAsAurApGbBoSFxCacKrgCDPOXW0W0h_2Ce0WsE4JZocQVb4qqjGqxQEL6D-QtGA6rJ8XS9o0VhnrtC5EH-Ey7_n-hip7sv0b5-VRZ3uUKV1WItrwGJrHgjV70GKo9o6xteldEl
link.rule.ids 315,786,790,4043,27956,27957,27958,41116,41558,42185,42627,52146,52269
linkProvider Springer Nature
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV07T8MwELagFYKFN2p5emACpYrjPMcKtRT6UEXTqmWJYscGhJRUfQzl13POY4DC0CkeEie-u9x9Z98DoVuV6yk8IjTORaSZUjLNJY7QwFWIhBtxQUO139Ht2a2h-Ty2xnke97yIdi-OJFNNnfqVOlU5vSAsHthj5UPoKlm9bIKBskqoXH-ctBuFAgaMTDPU69maeiA7zPx7kh_maFuGydqJaGpomgfILz4xiy_5rC0XrMa_flVv3HANh2g_B564nknKEdoS8THayVpRrmD0mqSjE-R3i465OMri8FQlZ5xIPE0AmSa4iN-K51jt4uL-aILDOML9zssIsxX-UCknCX-fJYCG84rYp2jQbPgPLS3vvaBxw9GJJqPIsChoZfBoCPOokEJ6OmXgskkSUlNI4gpOHN1zmQ0cdi2D2cBci-shN-gZKsVJLCoIe4zbJgGBoELVwTFC4hBmSLgCEHKlU0V3BQOCaVZgIwDHRJErWCNXFVWARUH4BgowGA4MVR5PZTyaBsxzX5A8yH_D-f8TnW909w3abfndTtB56rUv0J56bbYLc4lKi9lSXAEuWbDrXA6_Ad1q1BA
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07T8MwELZ4CMTCG7U8PTCBDLGd54ig5VFAFbQIWELs2ICQkqotA_x6znU88BoQUzwkTuI729-d775DaNvkeqqEKiKlyomvtSAxjRQBUyFXcS4Vz4y_4-IyPOn6Z7fBbVXndOCi3d2RpM1pMCxNxXC_l-v9kY3pcZPfC4qTwN5s7AnPJK5P-jBrQcUnD47vWg23GANe5hYBJyExD9iDzZ87-bQ1jeus_HY6Otp0mnPowX2ujTV52Xsdij35_oXJ8R__M49mK0CKD6wGLaAxVSyiKVui8g1a9-WotYQ6F66SLs5tfJ5heMalxr0SEGuJXVxXMcDGu4vbN3c4K3LcPr-6weINP5tUlFI-9UtAyRVT9jK6bjY6hyekqslAJIs8SnSes4DDag2WDhUJV1rpxOMCTDlNM-4rTWMlaeQlsQhB8nHARAhCD6SXScZX0ERRFqqGcCJk6FNQFK4MPw7LaEQF03AFgBTrqI52nDDSniXeSMFgMcOVfhuuOqqBuNLsERbGtHvNDG2eyYT0GfSz64Y_rabn4PeOVv909xaabh810_PTy9YamjFvtc6ZdTQx7L-qDYArQ7FZqeQHMIvc6w
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+diagnostics+of+potato+infections+with+PVY+and+PLRV+by+immunochromatography&rft.jtitle=Moscow+University+biological+sciences+bulletin&rft.au=Kondakova%2C+O.+A&rft.au=K.+O.+Butenko&rft.au=E.+V.+Skurat&rft.au=Yu.+F.+Drygin&rft.date=2016&rft.pub=Allerton+Press&rft.issn=0096-3925&rft.eissn=1934-791X&rft.volume=71&rft.issue=1&rft.spage=39&rft.epage=44&rft_id=info:doi/10.3103%2FS0096392516010041&rft.externalDocID=US201600195427
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0096-3925&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0096-3925&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0096-3925&client=summon