Molecular diagnostics of potato infections with PVY and PLRV by immunochromatography

An immunochromatography test system has been developed for molecular diagnostics of the potato virus Y and PLRV infection. To increase a low yield of PLRV and raise antibodies against the PLRV antigen, chimerical virus was constructed comprising the PLRV coat protein and recombinant RNA of a tobamov...

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Bibliographic Details
Published inMoscow University biological sciences bulletin Vol. 71; no. 1; pp. 39 - 44
Main Authors Kondakova, O. A, K. O. Butenko, E. V. Skurat, Yu. F. Drygin
Format Journal Article
LanguageEnglish
Published New York Allerton Press 2016
Subjects
antibodies
antigens
Biochemistry
Biomedical and Life Sciences
Cell Biology
coat proteins
diagnostic techniques
DNA
genes
immunoaffinity chromatography
Life Sciences
Plant Sciences
Potato virus Y
potatoes
recombinant proteins
RNA
Virology
viruses
Zoology
immunochromatography
molecular diagnostics
infection
potato virus Y
potato leafroll virus
chimerical PLRV
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Summary:An immunochromatography test system has been developed for molecular diagnostics of the potato virus Y and PLRV infection. To increase a low yield of PLRV and raise antibodies against the PLRV antigen, chimerical virus was constructed comprising the PLRV coat protein and recombinant RNA of a tobamovirus, in which capsid protein gene was replaced by the PLRV coat protein gene. Binary vector containing the DNA copy of the recombinant RNA was infectious, and yield of the chimerical virus increased up to 800 times in comparison with the wild type PLRV. On the basis of experience in the development of the diagnostics of viral and viroid infections, rational tactics are proposed for the mass laboratory and field diagnosis of viral infections on the molecular level.
Bibliography:http://dx.doi.org/10.3103/S0096392516010041
ISSN:0096-3925
1934-791X
DOI:10.3103/S0096392516010041