Simultaneous Analysis of Four Standards of The Herbal Formula, DF-02, of Ephedra intermedia and Rheum palmatum, using by High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD)

The herbal formula, DF-02, consisting of Ephedra intermedia and Rheum palmatum are used for the treatment of the metabolic diseases such as obesity and liver fibrosis in Korean local clinics. We aimed to develop the simultaneous analytical conditions for four standards, (+)-pseudoephedrine (PSEP) an...

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Published inNatural product sciences Vol. 25; no. 2; pp. 111 - 114
Main Authors Choi, Seong Yeon, Jeong, Birang, Jang, Hyeon Seok, Lee, Jiho, Kwon, Yong Soo, Yoon, Yoosik, Shin, Soon Shik, Yang, Heejung
Format Journal Article
LanguageEnglish
Published 한국생약학회 2019
Subjects
약학
(+)-pseudoephedrine
aloe-emodin
chrysophanol
Ephedra intermedia
(?)-ephedrine
Rheum palmatum
HPLC
validation
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Summary:The herbal formula, DF-02, consisting of Ephedra intermedia and Rheum palmatum are used for the treatment of the metabolic diseases such as obesity and liver fibrosis in Korean local clinics. We aimed to develop the simultaneous analytical conditions for four standards, (+)-pseudoephedrine (PSEP) and (−)-ephedrine (EP) for E. intermedia, and aloe-emodin (AE) and chrysophanol (CP) for R. palmatum using HPLC-UV techniques. The validated conditions yielded the high precision (relative standard deviation (RSD) < 3.65%) and the recoveries (94 – 106%) using the calibration curves with high linearity (R2 > 0.9994). As a result, four standards of DF-02 were simultaneously determined under the developed method, which will be utilized for the quality control or evaluation of DF-02 and many herbal preparations containing E. intermedia and R. palmatum. KCI Citation Count: 0
ISSN:1226-3907
2288-9027
DOI:10.20307/nps.2019.25.2.111