Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

• Published in: Medicina (Kaunas, Lithuania) Vol. 45; no. 5; pp. 372 - 377
• Main Authors: Labanauskiene, Jūrate, Satkauskas, Saulius, Kirveliene, Vida, Venslauskas, Mindaugas, Atkocius, Vydmantas, Didziapetriene, Janina
• Format: Journal Article
• Language: English
• Published: Switzerland 01.01.2009
• Subjects: Animals; Cell Line, Tumor; Electrochemotherapy; Electroporation; Humans; Indoles; Microscopy, Fluorescence; Neoplasms - drug therapy; Organometallic Compounds; Photochemotherapy - methods; Porphyrins; Radiation-Sensitizing Agents
• ISSN: 1648-9144; 1648-9144
• DOI: 10.3390/medicina45050047

The aim of our study was to determine if electroporation could improve the efficacy of photodynamic tumor therapy. A disadvantage of photodynamic therapy is a slow and in some cases insufficient accumulation of photosensitizer in tumor tissue, which could restrict the achievement of an efficient dose. Under the action of electric pulses, cells undergo membrane electroporation, which results in an increased permeability to various exogenous molecules. In this study, murine hepatoma MH22A cells were exposed to light in vitro in the presence of a photosensitizer, either chlorin e6 or aluminum phthalocyanine tetrasulfonate, following electroporation. Accumulation of the photosensitizers was registered by fluorescence microscopy. Cell viability was determined by the MTT assay. Our results demonstrate that electroporation improves an access of chlorin e6 and aluminum phthalocyanine tetrasulfonate to MH22A cells. Electroporation in combination with photosensitization significantly reduces viability of the treated cells even at low doses of photosensitizers.