Dominant Factors Affecting the Chromatographic Behaviour of Bile Acids

With the aim of optimizing the chromatographic process by avoiding any preliminary derivatizing step, we examined the chromatographic behaviour of a selected set of unconjugated bile acids looking at the dominant factors that affect the performances of three different stationary phases: RP-8, RP-18...

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Bibliographic Details
Published inChromatographia Vol. 64; no. 5-6; pp. 343 - 349
Main Authors Natalini, B, Sardella, R, Camaioni, E, Natalini, S, Pellicciari, R
Format Journal Article
LanguageEnglish
Published Vieweg Verlag 01.09.2006
Subjects
bile acids
buffers
chromatography
separation
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Summary:With the aim of optimizing the chromatographic process by avoiding any preliminary derivatizing step, we examined the chromatographic behaviour of a selected set of unconjugated bile acids looking at the dominant factors that affect the performances of three different stationary phases: RP-8, RP-18 and RP-18 Base Deactivated (RP-18-BD). Accordingly to its structural peculiarity, the RP-18-BD column combined with a specific mobile phase has proved to be the most suitable one, in enhancing both separation factor α and resolution R S within the selected set of analytes. Pronounced changes in the chromatographic profiles by only slightly changing the mobile phase composition (pH, buffer concentration, percentage and kind of organic modifier) prompted us to achieve satisfactory results in the separation and resolution of the selected set of bile acids.
Bibliography:http://dx.doi.org/10.1365/s10337-006-0013-9
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-006-0013-9