Mapping of leaf rust resistance genes in common wheat ‘Guinong08-6’

• Published in: Semina. Ciências agrárias : revista cultural e científica da Universidade Estadual de Londrina Vol. 44; no. 2; pp. 601 - 612
• Main Authors: Li, Luhua, Sui, Jianshu, Ren, Mingjian, Xu, Ruhong
• Format: Journal Article
• Language: English
• Published: Universidade Estadual de Londrina 01.03.2023
• Subjects: Complementary dominant genes; Puccinia triticina; Resistance gene; SSR
• ISSN: 1676-546X; 1679-0359
• DOI: 10.5433/1679-0359.2023v44n2p601

Wheat leaf rust (Puccinia triticina Eriks.), a devastating disease of wheat in the world, causes severe yield losses and therefore the development of resistant cultivars is very important. Here, a Chinese wheat line (Guinong08-6) showed adult-plant resistance against mixed fungal isolates of leaf rust, which is common in Guiyang region. It was crossed with a susceptible wheat line (Guinong19) to develop F1, F2, and F3 hybrids. Combined SSR and STS markers were used to map leaf rust resistance genes in Guinong08-6, and the resistance phenotype of Guinong08-6 was co-regulated by two complementary dominant genes, named LrGn08-6A and LrGn08-6B. LrGn08-6A was mapped to chromosome 2AS with markers URIC-LN2 and Xgpw2204, which flanked the gene at distances of 1.8 centimorgan (cM) and 14.83 cM, respectively. LrGn08-6B was mapped to chromosome 4DL with markers Xgpw342 and Xbarc93, which both flanked the gene at a distance of 26.57 cM. Genetic and molecular marker analyses demonstrated that LrGn08-6A, which was inherited from Aegilops ventricosa may be the resistance gene Lr37, while LrGn08-6B may be a newly discovered leaf rust resistance gene.