Characterization of a cDNA encoding an alpha thyroid hormone receptor in muscovy duckling

• Published in: Poultry science Vol. 75; no. 12; pp. 1531 - 1535
• Main Authors: Lachuer, J. (Universite Claude Bernard, Villeurbanne, Frane.), Ronfort, C, Duchamp, C, Cohen-Adad, F, Barges, S, Faraut, P, Quivet, L, Legras, C, Verdier, G, Barre, H
• Format: Journal Article
• Language: English
• Published: England 01.12.1996
• Subjects: ADN; Amino Acid Sequence; ANIMALES JOVENES; Animals; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Blotting, Northern; CANARD; Chickens; Cloning, Molecular - methods; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; DNA Primers; DNA, Complementary; Ducks; EXPRESION GENICA; EXPRESSION DES GENES; FOIE; HIGADO; HORMONAS DE LA TIROIDE; HORMONE THYROIDIENNE; JEUNE ANIMAL; Liver - metabolism; Molecular Sequence Data; MUSCLE; Muscle, Skeletal - metabolism; MUSCULOS; PATO; Polymerase Chain Reaction; RECEPTEUR D'HORMONE; RECEPTORES DE HORMONAS; Receptors, Thyroid Hormone - biosynthesis; RNA, Messenger - biosynthesis; SECUENCIA NUCLEOTIDICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Transcription, Genetic
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.0751531

A complementary deoxyribonucleic acid (cDNA) clone encoding an a thyroid hormone receptor (TRalpha) from muscovy duckling liver was isolated and sequenced. Comparison with the chicken TRalpha sequence showed a high degree of homology. Despite 45 nucleotide substitutions, the deduced peptide sequence was similar. This cDNA was used as a probe to characterize the TRalpha mRNA transcripts expressed in muscovy duckling liver and skeletal muscle