Analysis of Azithromycin in Human Plasma by LC-MS-MS

• Published in: Chromatographia Vol. 66; no. S1; pp. 115 - 118
• Main Authors: Yüzüak, N, Özden, T, Eren, S, Toptan, S
• Format: Journal Article
• Language: English
• Published: Wiesbaden : Vieweg Verlag 01.09.2007
• Subjects: antibiotics; Azithromycin and roxithromycin in human plasma; Column liquid chromatography-tandem mass spectrometry; Liquid-liquid extraction
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-007-0294-7

A simple sensitive and selective liquid chromatographic-tandem mass spectrometric method was developed and validated for the quantification of azithromycin in human plasma. Roxithromycin was used as the internal standard. The chromatographic separation was performed on a SunFire C18, 50 mm x 2.1, 3.5 μm column at 30 °C with mobile phase consisted of 1.54 g ammonium acetate, 250 mL water, 570 mL acetonitrile, 180 mL methanol and 0.6 mL glacial acetic acid. Flow rate was 0.2 mL min-¹. The work-up procedure involved a liquid-liquid extraction of the compounds. Mass spectrometric data were acquired in single ion monitoring. MRM mode of the ions 749.58 > 591.6 and 837.64 > 158.2 for azithromycin and roxithromycin, respectively. The method was validated in the concentration range of 2-1,000 ng mL-¹. Absolute recovery of azithromycin was 81.97%. Retention time for azithromycin was 0.9 and 1.1 min for roxithromycin. The run time was 2 min. This method was found suitable to analyse human plasma samples for application in pharmacokinetic, pharmacodynamic, bioavailability/bioequivalance studies.