PhePLATZ1, a PLATZ transcription factor in moso bamboo (Phyllostachys edulis), improves drought resistance of transgenic Arabidopsis thaliana

• Published in: Plant physiology and biochemistry Vol. 186; pp. 121 - 134
• Main Authors: Zhang, Kaimei, Lan, Yangang, Wu, Min, Wang, Linna, Liu, Hongxia, Xiang, Yan
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.09.2022
• Subjects: ABA signaling; Drought tolerance; Moso bamboo; PhePLATZ1; Stomatal closure; ABA signaling; PhePLATZ1; Moso bamboo; Stomatal closure; Drought tolerance
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2022.07.004

Drought is one of the most serious environmental stresses. Plant AT-rich sequence and zinc-binding (PLATZ) proteins perform indispensable functions to regulate plant growth and development and to respond to environmental stress. In this present study, we identified PhePLATZ1 in moso bamboo and found that its expression was up-regulated in response to 20% PEG-6000 and abscisic acid (ABA) treatments. Next, transgenic PhePLATZ1-overexpressing Arabidopsis lines were generated. Overexpression of PhePLATZ1 improved drought stress resistance of transgenic plants by mediating osmotic regulation, enhancing water retention capacity and reducing membrane and oxidative damage. These findings were corroborated by analysing physiological indicators including chlorophyll, relative water content, leaf water loss rate, electrolyte leakage, H2O2, proline, malondialdehyde content and the enzyme activities of peroxidase and catalase. Subsequent seed germination and seedling root length experiments that included exposure to exogenous ABA treatments showed that ABA sensitivity decreased in transgenic plants relative to wild-type plants. Moreover, transgenic PhePLATZ1-overexpressing plants promoted stomatal closure in response to ABA treatment, suggesting that PhePLATZ1 might play a positive regulatory role in the drought resistance of plants via the ABA signaling pathway. In addition, the transgenic PhePLATZ1-OE plants showed altered expression of some stress-related genes when grown under drought conditions. Taken together, these findings improve our understanding of the drought response of moso bamboo and provide a key candidate gene for the molecular breeding of this species for drought tolerance. •The expression level of PhePLATZ1 in moso bamboo was induced by drought and ABA.•Overexpression of PhePLATZ1 improved drought stress tolerance of Arabidopsis.•Transgenic Arabidopsis showed the decreased ABA-sensitivity during seed germination and early seedling growth.•Transgenic Arabidopsis promoted stomatal closure in response to ABA treatment.•PhePLATZ1 regulated the expression of several stress-related genes under drought stress.