Selection of nutritive compounds to improve growth and indicative properties of the DTM-Expert medium for dermatophytic fungi

• Published in: IOP conference series. Earth and environmental science Vol. 839; no. 2; pp. 22058 - 22063
• Main Authors: Savinov, V A, Ovchinnikov, R S, Gaynullina, A G, Khabarova, A V, Kapustin, A V
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.09.2021
• Subjects: Carbon sources; Fungi; Mannitol; Optimization; pH effects; Phenols
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/839/2/022058

Abstract The study describes optimization stages of Russian medium “DTM-Expert” for express detection of dermatophytic fungi. In order to improve the growth promotion and indicative properties of the medium, several variations of nutritive compounds were studied. The medium containing mannitol and glucose as carbon sources showed the best indicative and growth promotion properties. The pH optimum for dermatophytes growth on the DTM-Expert was in the range 4.7-5.5. The indicative properties of the medium were optimal at an initial pH value of 4.8. No significant differences were found when using bromothymol blue as an indicator compared to phenol red. The diagnostic efficiency of the optimized version of the DTM-Expert was 100% while the percentage of false positive results was 5.6%. The efficiency of the control medium was 87% accompanied by 9.2% of false positive reactions. The average reddening time was 10 days on the DTM-Expert and 11 days on the control medium.