Determination of Plasma Theophylline Concentration by Immuno-precipitation Inhibition Technique (i-PiT System)

• Published in: Byōin yakugaku Vol. 9; no. 4; pp. 295 - 300
• Main Authors: MOTOYAMA, SHIGERU, KOBAYASHI, JOJI, HAYATA, MICHIHARU, YOSHIMURA, SHOICHIRO, HORIBA, MICHIAKI, ITO, MASASHI, SUZUKI, KIYOSHI
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society of Pharmaceutical Health Care and Sciences 1983
• Subjects: immuno-precipitation inhibition technique (i-PiT system); plasma theophylline concentration
• ISSN: 0389-9098; 2185-9477
• DOI: 10.5649/jjphcs1975.9.295

Plasma theophylline concentration was determined by immuno-precipitation inhibition technique (i-PiT system). Immuno-reaction is dependent on incubation time and temperature, and therefore it is necessary to optimize the reaction. Within-day and between-day variances (C. V.) of analysis on 3 levels were less than 10%. The results of dilution test and recovery test were good. Forty-two plasma specimens of patients receiving theophylline therapy were studied by i-PiT system and high-pressure liquid chromatography (HPLC). The correlation coefficient was 0.988 and the regression line gave y= 0.978 x+0.918 (y: i-PiT, x: HPLC).