Identification of endophytic and rhizosphere bacteria in rice (Oryza sativa L.) in the experimental field at Payakumbuh State Agriculture Polytechnic, West Sumatra, Indonesia

Abstract The decrease of soil fertility and fewer soil microorganisms will lower crop production, particularly rice, thus threatening the national food security program. This study is (a) to isolate and identify the bacteria in the endophytic and rhizosphere of rice plants (b) to study the bacteria...

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Published inIOP conference series. Earth and environmental science Vol. 883; no. 1; pp. 12085 - 12092
Main Authors Sondang, Y, Siregar, R, Anty, K
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.10.2021
Subjects
Agriculture
Bacteria
Cereal crops
Crop production
Endophytes
Food security
Microorganisms
Plant growth
Plant tissues
Random sampling
Rhizosphere
Rice
rRNA 16S
Sampling
Sampling methods
Soil fertility
Soil layers
Soil microorganisms
Statistical sampling
West Sumatra Indonesia
Sumatra
Indonesia
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Summary:Abstract The decrease of soil fertility and fewer soil microorganisms will lower crop production, particularly rice, thus threatening the national food security program. This study is (a) to isolate and identify the bacteria in the endophytic and rhizosphere of rice plants (b) to study the bacteria from the endophytic and rhizosphere of rice plants which potentially stimulate plant growth. The experiment was carried out at the Laboratory of Food Crop Cultivation at Payakumbuh State Agriculture Polytechnic, Limapuluh Kota Regency, West Sumatra for four months. The sampling method was carried out by random sampling at rice planting in the Payakumbuh State Agriculture Polytechnic Experimental Field. Endophytic bacteria were taken from the root tissue of rice plants, and rhizosphere bacteria were taken from a layer of soil around rice roots. Isolation of bacteria was carried out by using the pour plate and scratchplate methods. Four bacteria were identified using the 16S rRNA sequencing method. The identification results showed that in the rice root tissue found the bacteria Chromobacterium rhizoryzae and Brevibacillus brevis . In the rice rhizosphere, Bacillus pseudomycoides and Bacillus thuringiensis are found. Bacteria are dominated by the Bacillus genera which can stimulate plant growth.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/883/1/012085