Associations between Lactoferrin (LTF) gene polymorphism exon 4 and milk compositions in Senduro goat

This research aimed to analyze the polymorphism of Lactoferrin (LTF) gene exon 4 associated with milk compositions in Senduro goats. A total of 42 DNA samples and milk compositions from Senduro goats were used in this study. The DNA sequence was amplified using Polymerase Chain Reaction (PCR) with a...

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Bibliographic Details
Published inE3S Web of Conferences Vol. 335; p. 43
Main Authors Susilorini, Tri Eko, Ridhowi, Aswah, Septian, Wike Andre, Furqon, Ahmad
Format Journal Article Conference Proceeding
LanguageEnglish
Published Les Ulis EDP Sciences 01.01.2022
Subjects
Alleles
Composition
Deoxyribonucleic acid
DNA
DNA sequencing
Gene polymorphism
Genotypes
Genotyping
Lactoferrin
Milk
Nucleotide sequence
Polymerase chain reaction
Polymorphism
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Summary:This research aimed to analyze the polymorphism of Lactoferrin (LTF) gene exon 4 associated with milk compositions in Senduro goats. A total of 42 DNA samples and milk compositions from Senduro goats were used in this study. The DNA sequence was amplified using Polymerase Chain Reaction (PCR) with a pair of primers. Genotyping was carried out using DNA sequencing and analyzed using FinchTV 1.4.0 and MEGA 6.0. In this research, the results showed that there were three genotypes (CC, CT, and TT) and two alleles (C and T). The frequencies of CC, CT, and TT genotypes were 0.381; 0.452; and 0.167, respectively. Furthermore, the frequencies of C and T alleles were 0.607 and 0.393, respectively. The genotype polymorphism did not affect on milk compositions. In conclusion, there was no association between polymorphism of LTF gene exon 4 and milk compositions in Senduro goats.
ISSN:2267-1242
2555-0403
2267-1242
DOI:10.1051/e3sconf/202233500043