Characterization of Prodiginine Pathway in Marine Sponge-Associated Pseudoalteromonas sp. PPB1 in Hilo, Hawai‘i

• Published in: Frontiers in sustainable food systems Vol. 4
• Main Authors: Sakai-Kawada, Francis E., Ip, Courtney G., Hagiwara, Kehau A., Nguyen, Hoang-Yen X., Yakym, Christopher-James A. V., Helmkampf, Martin, Armstrong, Ellie E., Awaya, Jonathan D.
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 18.12.2020
• Subjects: marine bacteria; marine sponge; pigments; prodiginine; prodigiosin; pseudoalteromonas
• ISSN: 2571-581X; 2571-581X
• DOI: 10.3389/fsufs.2020.600201

Interest in bioactive pigments stems from their ecological role in adaptation, as well as their applications in various consumer products. The production of these bioactive pigments can be from a variety of biological sources, including simple microorganisms that may or may not be associated with a host. This study is particularly interested in the marine sponges, which have been known to harbor microorganisms that produce secondary metabolites like bioactive pigments. In this study, marine sponge tissue samples were collected from Puhi Bay off the Eastern shore of Hilo, Hawai‘i and subsequently were identified as Petrosia sp. with red pigmentation. Using surface sterilization and aseptic plating of sponge tissue samples, sponge-associated microorganisms were isolated. One isolate (PPB1) produced a colony with red pigmentation like that of Petrosia sp., suggesting an integral relationship between this particular isolate and the sponge of interest. 16S characterization and sequencing of PPB1 revealed that it belonged to the Pseudoalteromonas genus. Using various biological assays, both antimicrobial and antioxidant bioactivity was shown in Pseudoalteromonas sp. PPB1 crude extract. To further investigate the genetics of pigment production, a draft genome of PPB1 was sequenced, assembled, and annotated. This revealed a prodiginine biosynthetic pathway and the first cited-incidence of a prodiginine-producing Pseudoalteromonas species isolated from a marine sponge host. Further understanding into the bioactivity and biosynthesis of secondary metabolites like pigmented prodiginine may uncover the complex ecological interactions between host sponge and microorganism.