Silencing of FAM111B inhibited proliferation, migration and invasion of hepatoma cells through activating p53 pathway

• Published in: Digestive and liver disease Vol. 55; no. 12; pp. 1679 - 1689
• Main Authors: Li, Feng, He, Hong-Ye, Fan, Zhi-Hao, Li, Chun-Ming, Gong, Yi, Wang, Xiao-Jun, Xiong, Hao-Jun, Xie, Chuan-Ming, Bie, Ping
• Format: Journal Article
• Language: English
• Published: Netherlands 01.12.2023
• Subjects: Carcinoma, Hepatocellular - pathology; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Epithelial-Mesenchymal Transition - genetics; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms - pathology; Tumor Suppressor Protein p53 - genetics; FAM111B; Proliferation; Migration; p53 pathway
• ISSN: 1590-8658; 1878-3562
• DOI: 10.1016/j.dld.2023.05.002

The function of Family with sequence similarity 111 member B (FAM111B) has been reported in multiple malignancies, but its involvement in occurrence and development of hepatocellular carcinoma (HCC) is still unclear. To investigate the role of FAM111B in HCC and explore the potential molecular mechanism. We examined the mRNA level of FAM111B via qPCR and protein level via immunohistochemistry in human HCC tissues. siRNA was used to construct a FAM111B-knockdown model in HCC cell lines. CCK-8, colony formation, transwell, and wound healing assays were performed to investigate the effect of FAM111B on proliferation, migration and invasion of HCC cell. Gene Set Enrichment Analysis, western blotting, and flow cytometry were carried out to find the related molecular mechanism. Human HCC tumor tissues exhibited higher expression of FAM111B, and high FAM111B expression was associated with poor prognosis. Vitro assays demonstrated that knockdown of FAM111B greatly repressed proliferation, migration and invasion of HCC cells. Furthermore, silencing of FAM111B significantly resulted in cell cycle arrest at G0/G1 and downregulation of epithelial-mesenchymal transition (EMT)-related proteins MMP7 and MMP9 via activation of p53 pathway. FAM111B played an essential role in promoting HCC development by regulation of p53 pathway.