The Three-Color FISH Method: A Comparison of Retrospective Cytogenetic Dose Estimations in Four Patients Who Underwent Acute Accidental Irradiation

• Published in: Biology bulletin of the Russian Academy of Sciences Vol. 50; no. 12; pp. 3278 - 3285
• Main Authors: Lomonosova, E. E., Nugis, V. Yu, Kozlova, M. G., Nikitina, V. A., Galstyan, I. A., Snigiryova, G. P.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.12.2023; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Cell Biology; Cytogenetics; Dental enamel; DNA probes; Ecology; Life Sciences; Lymphocytes; Peripheral blood; Radiation; Radiation Cytogenetics; Zoology; translocations; retrospective dose estimation; three-color FISH method; acute irradiation; peripheral blood lymphocyte culture
• ISSN: 1062-3590; 1608-3059
• DOI: 10.1134/S1062359023120166

This work presents the results of the use of three-color FISH-staining of chromosomes (DNA probes to pairs of chromosomes 1, 4, and 12) for the cytogenetic study of peripheral blood lymphocyte cultures of four patients at different times after irradiation in various emergency situations. All patients had initial dose estimations: one by EPR signal in the tooth enamel (1.12 Gy) and three by dicentric frequencies (1.9, 3.2, and 4.3 Gy). There was a strong significant linear correlation between the observed translocation frequencies and the initial dose estimations. Retrospective indication of the dose by translocation frequencies was carried out with the help of the dose–effect curve, obtained on the basis of experiments with the irradiation of blood of healthy donors in vitro. At the same time, the dose estimates in the long term after irradiation in three of these patients with the greatest lesions were significantly lower than they had been established earlier (1.26, 2.06, and 2.47 Gy). Coincidence of the initial and repeated results was observed only in one remaining patient.