In vitro Biofilm Formation by the Respiratory Mycoplasmosis Pathogen Mycoplasma pneumonia
This paper reports a study of biofilm formation by the Mycoplasma pneumoniae ( Mp ) strain Fh on an abiotic surface using light and electron microscopy. Quantitative assessment of the effectiveness of biofilm formation was carried out in polystyrene plates with crystal-Violet cell staining. To study...
Saved in:
Published in | Molecular genetics, microbiology and virology Vol. 34; no. 3; pp. 170 - 175 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.07.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | This paper reports a study of biofilm formation by the
Mycoplasma pneumoniae
(
Mp
) strain Fh on an abiotic surface using light and electron microscopy. Quantitative assessment of the effectiveness of biofilm formation was carried out in polystyrene plates with crystal-Violet cell staining. To study the ultrastructural organization of the
Mp
cells, scanning electron microscopy was used, which allowed a well-formed multicellular clearly defined structure of considerable size to be revealed by the third day of culturing in which
Mp
cells were submerged into the extracellular matrix with a clearly detectable system of pores. The morphology of the detected aggregations was absolutely identical to that of the biofilms described for other microorganisms, which confirmed that
Mp
had the ability to to form biofilms. The described phenomenon of the ability of
Mp
to grow in the form of the biofilm is of great importance both for understanding the causes of the long-term persistence of
Mp
in the human organism and for the development of new approaches for the treatment of protracted and chronic forms of infectious diseases caused by
Mp
. |
---|---|
ISSN: | 0891-4168 1934-841X |
DOI: | 10.3103/S0891416819030029 |