The Effect of Beta-Amyloid Peptides and Main Stress Protein HSP70 on Human SH-SY5Y Neuroblastoma Proteome

• Published in: Molecular biology (New York) Vol. 52; no. 6; pp. 937 - 946
• Main Authors: Rezvykh, A. P., Yurinskaya, M. M., Vinokurov, M. G., Krasnov, G. S., Mitkevich, V. A., Makarov, A. A., Evgen’ev, M. B., Zatsepina, O. G.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.11.2018; Springer Nature B.V
• Subjects: Actin; Alzheimer's disease; Apoptosis; Biochemistry; Biomedical and Life Sciences; Calcium-binding protein; Calmodulin; Cell culture; Cellular stress response; Chaperones; Cytoskeleton; Endoplasmic reticulum; Heat shock proteins; Hsp70 protein; Human Genetics; Life Sciences; Neuroblastoma; Peptides; Phosphorylation; Proteins; Proteomes; Proteomics; Tubulin; β-Amyloid; apoptosis; neuroblastoma SH-SY5Y; β-amyloid Аβ (1–42); HSP70; proteomics
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893318060158

The accumulation and aggregation of β-amyloids are major molecular events underlying the progression of Alzheimer’s disease. In neural cells, recombinant HSP70 reduces the toxic effect of Aβ and its isomeric forms. Here we describe the proteome of the neuroblastoma cell line after incubation with amyloid peptides Aβ42 and isomerized Asp7 (isoAβ42) without and with human recombinant heat shock protein 70 (HSP70). Incubation of SH-SY5Y cell culture with the synthetic Aβ-peptides leads to a decrease in the levels of several cytoskeleton proteins (e.g., ACTN1, VIME, TPM3) and several chaperonines involved in the folding of actin and tubulin (TCPQ, TCPG, TCPE, TCPB). These changes are accompanied by an increase in the expression of calmodulin and the proteins involved in folding in the endoplasmic reticulum and endoplasmic cell stress response. The presence of exogenous HSP70 has led to an increase in expression of several chaperones and a few other proteins including endogenous HSP70. A combined effect of recombinant HSP70 with Aβ peptides reduced cell apoptosis and significantly decreased the level of tubulin phosphorylation caused by the addition of Aβ peptides.