Development of Tumor-Induced Bystander Effect and Radiosensitivity in the Peripheral Blood Lymphocytes of Glioblastoma Patients with Different MGMT Gene Methylation Statuses in Tumor Cells

• Published in: Cytology and genetics Vol. 55; no. 2; pp. 125 - 131
• Main Authors: Zemskova, O. V., Kurinnyi, D. A., Rushkovsky, S. R., Demchenko, O. M., Romanenko, M. G., Glavatsky, O. Ya, Klymenko, S. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.03.2021; Springer Nature B.V
• Subjects: Apoptosis; Biomedical and Life Sciences; Biomedicine; DNA methylation; Glioblastoma; Glioblastoma cells; Human Genetics; Ionizing radiation; Lymphocytes; Peripheral blood; Radiosensitivity; Statistical analysis; Tumor cells; glioblastoma; methylation; Comet assay; tumor-induced bystander effect
• ISSN: 0095-4527; 1934-9440
• DOI: 10.3103/S0095452721020158

The effect of the MGMT gene methylation in glioblastoma cells on the development of the tumor-induced bystander effect and the modification of sensitivity, due to the impact of ionizing radiation, in the untransformed bystander cells (peripheral blood lymphocytes) was studied. The obtained results showed development of a glioblastoma-induced bystander effect. No association between the manifestation of the tumor-induced bystander effect and the methylation status of the MGMT gene promoter region in tumor cells has been found. After the exposure to radiation, statistically significant ( p < 0.01) changes were observed in the apoptotic activity and radiosensitivity of peripheral blood lymphocytes with different statuses of the MGMT gene methylation in the tumor cells of glioblastoma patients.