Recombinase Polymerase and Loop-Mediated Isothermal Amplification in the DNA Diagnostics of Infectious Diseases

Recombinase polymerase and loop-mediated isothermal amplification can be conducted in nonlaboratory conditions, making these methods promising for the development of rapid tests for the DNA diagnosis of infectious diseases in humans, agricultural animals, and plants in the “point-of-care” testing or...

Full description

Saved in:
Bibliographic Details
Published inJournal of analytical chemistry (New York, N.Y.) Vol. 79; no. 3; pp. 273 - 286
Main Authors Kurbatov, L. K., Ptitsyn, K. G., Khmeleva, S. A., Radko, S. P., Lisitsa, A. V., Suprun, E. V.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.03.2024
Springer Nature B.V
Subjects
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Colorimetry
Infectious diseases
Principles
Reviews
DNA diagnostics
CRISPR/Cas nuclease
noninstrumental analysis
infectious disease
isothermal amplification
Online AccessGet full text

Cover

More Information
Summary:Recombinase polymerase and loop-mediated isothermal amplification can be conducted in nonlaboratory conditions, making these methods promising for the development of rapid tests for the DNA diagnosis of infectious diseases in humans, agricultural animals, and plants in the “point-of-care” testing or “in-field” detection format. The review discusses the fundamental principles underlying these methods and describes their current status, with a focus on noninstrumental methods for the detection of results in isothermal amplification using colorimetry and lateral flow assays. Approaches to enhancing the selectivity of isothermal amplification through its combination with CRISPR/Cas detection or by combining two methods based on the “nested amplification” principle are thoroughly examined.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934824030080