Physiological Response of Avena sativa to Low-Temperature Stress is Promoted by Bacillus amyloliquefaciens GL18 and Its Functional Genes

• Published in: Russian journal of plant physiology Vol. 69; no. 7
• Main Authors: Chen, L., Xie, Y. L., Wu, X. H., Yang, X., Wang, T., Peng, W. X.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.12.2022; Springer Nature B.V
• Subjects: Abscisic acid; Accumulation; Acetic acid; Avena sativa; Bacillus; Bacillus amyloliquefaciens; Biomass; Biomedical and Life Sciences; Catalase; Gene clusters; Genes; Genomes; Hormones; Hydrogen peroxide; Indoleacetic acid; Irrigation; Jasmonic acid; Life Sciences; Low temperature; Peroxidase; Physiology; Plant growth; Plant Physiology; Plant Sciences; Polyamines; Research Papers; Salicylic acid; Seedlings; Strain; Superoxide dismutase; Surfactin; Synthesis; stress physiology; functional gene analysis; response to low-temperature stress
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443722601586

Low temperature affects plant growth and biomass accumulation in the Qinghai-Tibet Plateau. Bacillus is an important plant growth-promoting rhizobacteria that can promote plant growth directly or indirectly. In this study, bacteria suspensions (cell concentration 1 × 10 7 cfu/mL) of Bacillus amyloliquefaciens strain GL18 were interacted with Avena sativa seedlings by the root irrigation method under 4°C low-temperature conditions. The biomass accumulation, as well as the physiological and biochemical activities of A. sativa , was detected over 14 days. The results showed that the plant height, root length, fresh and dry weight of A. sativa treated at 4°C with B. amyloliquefaciens by root irrigation increased by 39.28, 32.50, 114.89, and 87.17%, respectively, compared to untreated control. The levels of plant hormones such as abscisic acid, salicylic acid and jasmonic acid were significantly increased, and the activities of superoxide dismutase, peroxidase and catalase increased rapidly and then decreased slowly. The accumulation of hydrogen peroxide and malondialdehyde decreased, and the contents of proline and betaine increased significantly. The genome of GL18 strain was sequenced and its related functional genes were analyzed. The results showed that genome of GL18 strain contained gene clusters encoding proteins related to the synthesis of lipopeptide compounds such as surfactin, iturin, and fengycin, encoding genes involved in 3-indole acetic acid synthesis as well as genes related to the synthesis of plant growth-promoting substances such as siderophore and polyamines. This study provided experimental data and a theoretical basis for the manner in which Bacillus promotes the growth of A . sativa under low-temperature stress.