Genetic Effects of Indica Lineage Introgression on Amylopectin Chain Length Distribution in Japonica Milled Rice

The fine structure of amylopectin affects rice quality; in particular, the amylopectin chain length distribution (ACLD) in milled rice differs between subspecies of Oryza sativa L. However, the correlation between ACLD and quality trait factors, and the genetic basis of ACLD phenotypic variation, ar...

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Bibliographic Details
Published inAgriculture (Basel) Vol. 12; no. 4; p. 472
Main Authors Cui, Juan, Zhao, Xue, Yu, Yuejiao, Zhang, Wenxing, Kong, Ximan, Sun, Jian, Chen, Wenfu
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.04.2022
Subjects
Amylopectin
amylopectin chain length distribution
Chains
Chromosome 11
Chromosome 3
Chromosomes
Cooking
Correlation analysis
Eating quality
Enzymes
Fine structure
genetic background
Genetic effects
Genomes
indica lineage
Oryza sativa indica
Oryza sativa japonica
Phenotypic variations
Plant breeding
quality trait factors
Rice
RVA
Segments
Ultrastructure
Viscosity
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Summary:The fine structure of amylopectin affects rice quality; in particular, the amylopectin chain length distribution (ACLD) in milled rice differs between subspecies of Oryza sativa L. However, the correlation between ACLD and quality trait factors, and the genetic basis of ACLD phenotypic variation, are still unknown. Here, the correlations of ACLD with cooking and eating quality and with the rapid viscosity analysis (RVA) index were studied using chromosome segment substitution lines (CSSLs). Clear variations in ACLD were observed in introgression lines: introgression of indica segments of chromosome 3 and 7 increased the proportion of amylopectin Fa, and another segment of chromosome 3 reduced the proportion of amylopectin Fb2. A segment of chromosome 11 decreased the proportion of amylopectin Fa but increased that of Fb3. Correlation analysis with the RVA index further showed that the breakdown viscosity (BDV) was negatively correlated with the proportion of amylopectin Fb1, Fb2, and Fb3 chains, and positively correlated with Fa. Consistency viscosity (CSV) values were negatively correlated with the proportion of amylopectin Fb1, Fb2, and Fb3 chains. We thus clarified the quality trait factors determined by variation in ACLD, and provide key information for pyramiding inter-subspecific genetic superiority in molecular design breeding for rice quality.
ISSN:2077-0472
2077-0472
DOI:10.3390/agriculture12040472